葛根素对妊娠期糖尿病大鼠母体和胎儿的影响

Effects of puerarin on maternal gestational diabetes mellitus rats and fetal growth

目的观察口服葛根素(puerarin,Pue)对妊娠期糖尿病(gestational diabetic mellitus,GDM)大鼠母体的药效及对其胎儿生长发育的影响,为Pue用于治疗GDM提供参考。方法给已孕母鼠尾静脉注射链脲佐菌素建立GDM大鼠模型,灌胃Pue治疗12 d,记录孕鼠体质量及流产情况,检测孕鼠给药治疗前后的空腹血糖,并分别于给药后的第5天和第10天检测母鼠糖耐量;母鼠怀孕第20天行剖腹产,检测胎鼠血糖含量,并观察胎鼠生长发育情况。测定胎鼠体质量、体长、尾长及胎盘和重要脏器的重量,计算脏器指数。结果与模型组相比,Pue可显著降低GDM孕鼠及胎鼠的空腹血糖,改善孕鼠糖耐量,有效缓解GDM导致的孕鼠体质量过度增加和胎鼠体质量过大的状况,降低流产率;并可逆转GDM引起的胎鼠脑、心、肝等脏器指数下降和肾脏脏器指数升高。结论口服Pue可缓解GDM母体及胎儿的高血糖状态,降低流产率,减少巨大儿的发生,促进胎儿重要脏器的发育。

Aim To observe the effects of oral puerarin(Pue)on the maternal gestational diabetes mellitus(GDM)rats and its fetal growth and development,so as to provide references for the application of Pue in the treatment of GDM.Methods The GDM rat model was established by injecting streptozotocin(STZ)into the tail vein of pregnant female rats,and the rats were treated with Pue orally for 12 days;the body weight and abortion of pregnant rats were recorded.The fasting blood glucose of pregnant rats was detected before and at the end of the treatment,and the glucose tolerance was tested on the 5th and 10th days after the administration of the drug.The cesarean sections were carried out on the 20th day of pregnancy.The blood glucose content of the fetal rats was detected,and the state of development was observed.The body weight and length were measured,as well as the placenta and the important organs weight,and the indexes of the organs were calculated.Results Compared with the model group,Pue could significantly reduce the fasting blood glucose of GDM pregnant rats and fetal rats,improve the glucose tolerance of pregnant rats,effectively alleviate the excessive weight gain of pregnant rats and overweight of fetal rats caused by GDM,and reduce the abortion rate;it could also reverse the decrease in the indexes of the organs of brain,heart,and liver,and the increase in the indexes of organs of kidney in fetal rats caused by GDM.Conclusions Pue can relieve the maternal and the fetal hyperglycemia in GDM,reduce the rate of miscarriage,reduce the incidence of macrosomia,and promote the development of vital fetal organs.