摘要
A large team led by German scientists at the University of G?ttingen has pushed optical microscopy past the milestone of single-nanometer resolution with a relatively inexpensive superresolution microscopy(SRM)technique called one-step nanoscale expansion(ONE),reporting their work in late 2022[1].The technique employs two widely used SRM strategies,expansion and tracking fluorescence fluctuations,combining a 10-fold axial specimen expansion(1000-fold by volume)with computational analysis of fluctuations in fluorescence across up to 3000 images of a single biological sample(Fig.1).
