番茄潜叶蛾雌雄成虫肠道可培养细菌多样性研究

Intestine culturable bacteria diversity of male and female adults of Tuta absoluta(Meyrick)

【目的】明确番茄潜叶蛾雌雄成虫肠道可培养细菌的组成结构及多样性,为番茄潜叶蛾肠道细菌多样性和功能的深入研究提供理论依据。【方法】采用传统分离培养法对番茄潜叶蛾雌雄成虫肠道可培养细菌进行分离培养,并根据菌株的菌落形态和16S rDNA序列进行种属鉴定。【结果】从番茄潜叶蛾雌成虫肠道中分离得到16株细菌,分属于2门7科12属,优势门、科、属、种分别为变形菌门(Proteobacteria)(84.30%)、肠杆菌科(Enterobacteriaceae)(83.79%)、肠杆菌属(Enterobacter)(83.38%)和成都肠杆菌(E.chengduensis)(81.86%),Brachybacterium ginsengisoli(6.32%)、耐盐节杆菌(Arthrobacter pascens)(2.04%)和B.vulturis(1.33%)为主要细菌种类;从番茄潜叶蛾雄成虫肠道中分离得到12株细菌,分属于2门3科6属,优势门、科、属、种分别是厚壁菌门(Firmicutes)(74.02%)、芽孢杆菌科(Bacillaceae)(73.36%)、芽孢杆菌属(Bacillus)(67.12%)和蜡样芽孢杆菌(B.cereus)(25.91%),Atlantibacter subterranea(21.64%)、花域芽孢杆菌(B.vallismortis)(17.48%)和嗜气芽孢杆菌(B.aerophilus)(14.05%)为主要细菌种类。【结论】从番茄潜叶蛾雌雄成虫肠道中共分离到3门9科18属27种细菌,其中在雌成虫肠道中分离到2门7科12属16种细菌,以肠杆菌属为优势菌属,在雄成虫肠道中分离到2门3科6属11种细菌,以芽孢杆菌属为优势菌属;雌雄成虫肠道中未分离到同种细菌。

【Objective】To clarify the composition structure and diversity of culturable bacteria in the intestine of male and female adults of Tuta absoluta(Meyrick),and to provide a theoretical basis for further study on the diversity and func-tion of intestine bacteria of T.absoluta.【Method】The traditional isolation and culture method was used to isolate and cul-ture the bacteria in the intestine of male and female adults of T.absoluta.And the species were identified by colony mor-phology and 16S rDNA sequence analysis.【Result】There were 16 strains of bacteria belonging to 2 phyla,7 families,12 genera and 16 species were isolated from the intestine of female adults.The dominant phylum,family,genus and species were Proteobacteria(84.30%),Enterobacteriaceae(83.79%),Enterobacter(83.38%)and E.chengduensis(81.86%).Brachybacterium ginsengisoli(6.32%),Arthrobacter pascens(2.04%)and B.vulturis(1.33%)were the main bacterial species.A total of 12 strains of bacteria belonging to 2 phyla,3 families male adults.The dominant phylum,family,genus and species were Firmicutes(74.02%),Bacillaceae(73.36%),Bacillus(67.12%)and B.cereus(25.91%).Atlantibacter subterranea(21.64%),B.vallismortis(17.48%)and B.aerophilus(14.05%)were the main bacterial species.【Conclusion】A total of 3 phyla,9 families,18 genera and 27 species of bacte-ria are isolated from the intestine of male and female adults of T.absoluta.Among them,2 phyla,7 families,12 genera and 16 species bacteria are isolated from the intestine of female adults and Enterobacter is the dominant bacterium;2 phyla,3 families,6 genera and 11 species bacteria are isolated from the intestine of male adults and Bacillus is the dominant bac-terium.No same kind of bacterium is isolated from the intestine of male and female adults.