离子淌度质谱法在D-氨基酸修饰多肽研究中的进展

Research Progress of Ion Mobility Mass Spectrometry in D-Amino Acid Containing Peptides

D-氨基酸修饰多肽(D-Amino Acid Containing Peptides,DAACPs)具有独特的稳定性和生物学活性,并已在包括哺乳动物在内的多种动物体内检测到。但因为检测手段的匮乏,可能仍有许多种类的DAACP还未被发现,所以开发高效的DAACPs的分析方法是很必要的。而离子淌度质谱因其独特的分离原理很适用于多肽外消旋化研究,本文概括了离子淌度质谱法对外消旋化前后多肽的分析策略,为DAACPs高效率的检出提供可能的方法,并综述了预测碰撞截面值(Collision Cross Section,CCS)的相关方法,总结了离子淌度质谱在外消旋化修饰位点鉴定及含量测定方面的优势,为离子淌度质谱法对DAACPs的分析提供参考。

D-Amino Acid Containing Peptides(DAACPs)have been detected in a variety of species including mammals,and have unique stability and biological activity.However,due to the lack of detection methods,there may still be many types of DAACPs that have not been discovered.Therefore,it is necessary to develop efficient detection methods for discovering and identifying DAACPs.Ion mobility mass spectrometry(IM-MS)is suitable for the study of peptide racemization due to its unique separation principle.This paper summarized the analysis strategy of peptides before and after racemization by IM-MS,and provided possible methods for the efficient detection of DAACPs.Also,the methods for predicting Collision Cross Section(CCS)were reviewed and described,and the advantages of IM-MS in the identification and content determination of racemic modification sites were summarized,so as to provide reference for the analysis of DAACPs by IM-MS.