弗尼斯弧菌引起的急性腹泻1例

A case of acute diarrhea caused by Vibrio furnissii

弗尼斯弧菌(Vibrio furnissii)是革兰氏阴性短小杆菌,多从腹泻病人粪便中分离得到。国内、外均少见对弗尼斯弧菌感染的相关报道。本文报道就诊于南宁市第二人民医院的1例急性腹泻患者感染弗尼斯弧菌病例,患者腹泻感染症状典型,表现为4年反复腹泻,7~8次/天。来自该病例的弗尼斯弧菌因部分生化反应与河流弧菌相似而难以区分,与质谱鉴定结果有所差异。最终本研究将通过16S rRNA测序后的基因组与相应的模式株基因进行平均核酸一致性(average nucleotide identity,ANI)的比对,将此病原菌准确鉴定为弗尼斯弧菌。本文主要介绍了弗尼斯弧菌的生长情况、实验室检测,并回顾性分析了国内外相关文献报道,这对于提高实验室人员对该菌的认识以及临床诊治少见弧菌引起的腹泻病有一定的指导意义。

Vibrio furnissii is a gram-negative short bacilli isolated from the feces of patients with diarrhea.Domestic and foreign reports of Vibrio furnissii infection are rare.This article reported a case of Vibrio furnissii infection in an acute diarrhea patient who was treated in the Second People’s Hospital of Nanning,and the patient’s diarrhea infection symptoms were typical,showing 4 years of repeated diarrhea,7-8 times per day.Vibrio furnissii from this case was difficult to distinguish from Vibrio fluvialis due to the similarity of the biochemical reaction,and the results were different from the mass spectrometry identification results.Finally,the genome after 16S rRNA sequencing was compared with the corresponding model strain gene for average nucleotide identity(ANI).This pathogen was accurately identified as Vibrio furnissii.The growth and laboratory detection of Vibrio furnissii were introduced,and the related literatures at home and abroad were retrospectively analyzed,which is of certain guiding significance to improve the understanding of laboratory personnel about the bacterium and the clinical diagnosis and treatment of diarrheal diseases caused by Vibrio furnissii.