大鼠胸主动脉钙化程度与血清多项指标表达的关系

Relationship between the degree of thoracic aorta calcification and the expression of several serum indexes in rats

目的 分析血清特征指标表达特征与大鼠胸主动脉钙化程度的相关性。方法 2020年3月-2021年5月,对40只健康大鼠和40只胸主动脉钙化大鼠(根据钙化严重程度分为轻度组25例、中度组7例和重度组8例)进行对照实验研究,比较健康组和模型组大鼠、模型组不同病情大鼠总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白、空腹血糖、碱性磷酸酶、全段甲状旁腺激素、白蛋白、血红蛋白、补体成分3、钙离子、磷、肌酐、尿酸、血尿素氮含量的统计学差异。采用SPSS 20.0统计软件,计量资料用均值±标准差表示,两组比较用两独立样本t检验,三组比较用F检验,两两比较用SNK-t检验。结果 模型组总胆固醇(t=11.792,P<0.001)、甘油三酯(t=14.598,P<0.001)、低密度脂蛋白(t=12.217,P<0.001)、碱性磷酸酶(t=42.948,P<0.001)、全段甲状旁腺激素(t=7.277,P<0.001)、补体成分3(t=26.223,P<0.001)含量均高于健康组,模型组的高密度脂蛋白(t=16.116,P<0.001)、白蛋白(t=19.728,P<0.001)、血红蛋白(t=12.846,P<0.001)含量低于健康组,差异均有统计学意义。不同病情大鼠比较,总胆固醇(F=39.778,P<0.001)、甘油三酯(F=13.068,P<0.001)、低密度脂蛋白(F=4.252,P<0.001)、高密度脂蛋白(F=6.213,P<0.001)、全段甲状旁腺激素(F=33.990,P<0.001)、白蛋白(F=10.271,P<0.001)、血红蛋白(F=4.291,P<0.001)、补体成分3(F=160.229,P<0.001)含量的差异均有统计学意义。结论 大鼠胸主动脉钙化程度与多项血清指标血脂指标及相关酶、蛋白、补体均存在一定关联性。

Objective To analyze the correlation between the expression characteristics of serum characteristic indexes and the degree of thoracic aorta calcification in rats.Methods From March 2020 to May 2021,a controlled experimental study was conducted on 40 healthy rats and 40 rats with calcification of the thoracic aorta(divided into a mild group with 25 cases,a moderate group with 7 cases,and a severe group with 8 cases according to the severity of calcification).The levels of total cholesterol(CHOL),triglyceride(TG),low density lipoprotein(LDL),high density lipoprotein(HDL),fasting blood glucose(FBG),alkaline phosphatase(ALP),intact parathyroid hormone(iPTH),albumin(Alb),hemoglobin(HGB),complement component 3(C3),calcium ion(Ca2*),phosphorus(P),creatinine(CRE),uric acid(UA),and blood urea nitrogen(BUN)were compared between the healthy group and the model group,and between the model group with different conditions.SPSS 20.O statistical software was used for statistical analysis,and the measurement data were represented by meantstandard deviation.The t-test of two independent samples was used for comparison of two groups,the F test was used for comparison of three groups,and the SNK-t test was used for pairwise comparison.Results The levels of CHOL(t=11.792,P<0.001),TG(t=14.598,P<0.001),LDL(t=12.217,P<0.001),ALP(t=42.948,P<0.001),iPTH(t=7.277,P<0.001),and complement component 3(t=26.223,P<0.001)in the model group were higher than those in the healthy group.The levels of HDL(t=16.116,P<0.001),Alb(t=19.728,P<0.001),and HGB(t=12.846,P<0.001)in the model group were lower than those in the healthy group,and the differences were statistically significant.Compared with rats with different disease conditions,the levels of CHOL(F=39.778,P<0.001),TG(F=13.068,P<0.001),LDL(F=4.252,P<0.001),HDL(F=6.213,P<0.001),iPTH(F=33.990,P<0.001),Alb(F=10.271,P<0.001),HGB(F=4.291,P<0.001),complement component 3(F=160.229,P<0.001)were statistically significant.Conclusion The degree of thoracic aorta calcification in rats is correlated with several serum indexes,lipid indexes,related enzymes,proteins,and complement.