纳米罗勒精油/聚乙烯吡咯烷酮-聚乙烯醇水凝胶伤口敷料制备及性能表征

Preparation and characterization of Basil essential oil nanoparticles/polyvinylpyrrolidone-polyvinyl alcohol hydrogel wound dressing

罗勒精油(Basil essential oil,BEO)是一种绿色、安全的抑菌剂。然而,BEO的强挥发性限制了其在抑菌伤口敷料领域的应用。本文采用纳米沉淀法制备了纳米罗勒精油(BEO@Zein(玉米醇溶蛋白)),然后将其负载在以聚乙烯吡咯烷酮(PVP)和聚乙烯醇(PVA)为基材的水凝胶上,通过冻融循环形成了BEO@Zein/PVPPVA水凝胶伤口敷料,对BEO@Zein和水凝胶的微观形貌和结构进行表征,对水凝胶的抑菌性能、力学性能、溶胀保湿性、降解性、血液相容性进行研究。结果表明:BEO@Zein形成了以BEO为核、Zein为壳的纳米球形结构(平均粒径为56.3 nm),显著降低了BEO挥发性。BEO@Zein/PVP-PVA水凝胶可以缓慢释放BEO,从而表现出优异的缓释抑菌性能。因此,BEO@Zein/PVP-PVA水凝胶具有良好的抑菌持久性(超过72 h)。此外,水凝胶还表现出显著的抗细菌生物膜性能。BEO@Zein/PVP-PVA水凝胶的力学性能、溶胀保湿性、降解性和血液相容性均表现良好。研究表明:BEO@Zein/PVP-PVA水凝胶是一种良好的伤口敷料材料。

Basil essential oil(BEO)is a green and safe antibacterial agent.However,the high volatility of BEO has limited its application in the field of antibacterial wound dressings.BEO nanoparticles(BEO@Zein)were prepared by nanoprecipitation method.They were then loaded on the hydrogel based on polyvinylpyrrolidone(PVP)and polyvinyl alcohol(PVA)to form BEO@Zein/PVP-PVA hydrogel wound dressing by freeze-thaw cycle.The microscopic morphology and structure of BEO@Zein and hydrogel were characterized.The antibacterial property,mechanical property,swelling and moisturizing,degradability and blood compatibility of hydrogel were studied.The results show that BEO@Zein forms a nano-spherical structure with BEO as core and Zein as shell(mean particle size is 56.3 nm),which significantly reduces the volatility of BEO.BEO@Zein/PVP-PVA hydrogel can release BEO slowly,thus exhibiting excellent slow-release antibacterial property.Therefore,BEO@Zein/PVP-PVA hydrogel has excellent antimicrobial persistence(over 72 h).In addition,the hydrogel shows remarkable antibacterial biofilm property.BEO@Zein/PVP-PVA hydrogel has good mechanical property,swelling and moisturizing,degradability,and blood compatibility.Studies have shown that BEO@Zein/PVP-PVA hydrogel can be used as a good wound dressing material.