蛛丝丝素蛋白膜的制备及其释药性能评价

Preparation and drug release evaluation of spider fibroin membrane

天然蜘蛛丝具有优越的力学性能,其多种剂型具有优异的抗菌活性、生物相容性及良好的导热性。开发具有良好生物相容性的纯天然生物载药材料,对于降低患者的强烈免疫原性反应具有重要意义。通过对蛛丝在不同溶剂中溶解及成膜方式的探索,优化反应条件:以六氟异丙醇做溶解溶剂,以1∶1(mg∶mL)的料液质量体积比进行投料,在60℃条件下溶解8 h,通过溶剂浇铸法成膜,并结合相关表征探索其以罗丹明B为模型药物的体外释药规律。通过浸提法测试材料细胞毒性,将蛛丝丝素蛋白膜浸提液与细胞共培养显示其对小鼠胚胎成骨细胞无潜在细胞毒性。制得的载药蛛丝丝素蛋白膜产率高、厚度均一、操作简单,为天然蜘蛛丝丝素蛋白的溶解及其膜剂的制备提供了一项简单易行的技术。

Natural spider silk has superior mechanical properties,and its various forms have excellent antibacterial activity,biocompatibility,and good thermal conductivity.It is of great significance to develop natural biological drugloaded materials with good biocompatibility for reducing the strong immunogenicity reaction of patients.By exploring the dissolution and membrane-forming methods of spider silk in different solvents,the reaction conditions were optimized,hexafluoroisopropanol was used as the dissolution solvent,the material ratio was 1∶1(mg∶mL),the spider silk was dissolved at 60℃for 8 h,and the membrane was formed by solvent casting,and the drug release in vitro of rhodamine B(RhB)as the model drug was explored with the correlation characterization.The cytotoxicity of the material was tested by extraction method.The spider fibroin membrane extract co-culture with cells showed that the material had no potential cytotoxicity to mouse embryonic osteoblasts cells.The drug-loaded spider silk fibroin membrane prepared by this method has high yield,uniform thickness and simple operation,which provides a simple and feasible technology for the dissolution of natural spider silk protein and the preparation of membrane agent.