基于转录组的瑶药半枫荷根和叶的差异分析

Differences between roots and leaves of Semiliquidambar cathayensis by transcriptiomic analysis

【目的】瑶药半枫荷(Semiliquidambar cathayensis)的根和叶均具有祛风通络等功效,但其黄酮类等活性成分存在差异。对半枫荷进行转录组测序,以期获得其根和叶的转录组信息和差异表达基因,对探究半枫荷根和叶的差异表达基因在活性成分合成通路中的表达规律具有重要意义。【方法】利用Illumina对半枫荷根和叶进行转录组测序,并进行生物信息分析以挖掘两者萜类和黄酮类等关键次生代谢产物合成通路的基因表达差异。【结果】59378个差异表达基因归类到GO分类的3个大类中,主要与生物学过程有关(50.59%)。185个差异表达基因注释KOG数据库的24个分类中。81个差异表达基因参与苯丙烷类化合物的生物合成,30个差异表达基因参与黄酮类化合物的生物合成,86个差异表达基因参与萜类化合物的生物合成。【结论】参与黄酮类化合物合成途径的关键酶CHS等均为上调表达,导致根的黄酮类成分多于叶,而CMS、HMGS等关键酶都可能影响半枫荷不同组织中萜类化合物的积累模式。文章获得了半枫荷根和叶的转录组信息特征,为今后半枫荷基因功能鉴定、次生代谢途径解析及调控机制研究提供依据。

[Objective]Both the roots and leaves of Semiliquidambar cathayensis have the effect of dispelling wind and removing obstruction in the meridians,but they are different in active components such as flavonoids.Transcriptomic sequencing was performed to obtain the transcriptome information and differentially expressed genes between roots and leaves of S.cathayensis.It is important to explore the expression of the key genes involved in the active component synthesis of S.cathayensis.[Methods]We carried out transcriptomic sequencing by Illumina high-throughput sequencing technology.Additionally,differences in gene expression for the synthetic pathways of the key secondary metabolites,such as terpenes and flavonoids,were identified and analyzed by bioinformatics analysis.[Results]59378 differentially expressed genes(DEGs)were classified into three major classes in GO classification,involving mainly in biological process functions(50.59%).185 DEGs were annotated in 24 categories of the KOG classification.81 differentially expressed genes were involved in phenylpropane biosynthesis,30 differentially expressed genes in flavonoid biosynthesis,and 86 differentially expressed genes in terpenoid biosynthesis.[Conclusion]The key enzymes involved in flavonoid synthesis,such as CHS,were up-regulated,resulting in more flavonoids in roots than leaves.The key enzymes such as CMS and HMGS may affect the accumulation patterns of terpenoids in different tissues of S.cathayensis.The transcriptomic data of roots and leaves of S.cathayensis were obtained,which laid a foundation for the study of functional identification as well as understanding the secondary metabolic pathway and regulatory mechanism of S.cathayensis.