血清IL-34表达水平与原发性胆汁性胆管炎的相关性分析

Correlation between serum IL-34 level and primary biliary cholangitis

目的:探讨IL-34是否参与原发性胆道胆管炎(PBC)发病机制。方法:将49例PBC患者与50例年龄、性别匹配的健康对照组进行比较,并对治疗前后进行比较。ELISA和化学发光法(CL)检测血清IL-34、抗线粒体抗体M2(AMA-M2)和部分细胞因子IL-1、IL-2、IL-6、IL-8、IL-10和TNF-α浓度。结果:PBC患者血清IL-34水平明显高于健康对照组(P<0.01)。IL-34水平与AMA-M2水平具有一定相关性。治疗有效患者中,血清IL-34水平明显降低。PBC患者血清IL-2、IL-6、TNF-α水平较健康对照组明显升高。结论:本研究为IL-34在PBC发展中的新作用以及作为治疗靶点的潜在用途提供了支持。血清IL-34可能是PBC的候选生物标志物。

Objective:To investigate whether IL-34 is involved in pathogenesis of primary biliary cholangitis(PBC).Methods:Forty-nine patients with PBC were compared with 50 age-and sex-matched healthy controls before and after treatment.Concentrations of serum IL-34,anti-mitochondrial antibody M2(AMA-M2)and some cytokines IL-1,IL-2,IL-6,IL-8,IL-10 and TNF-αwere detected by ELISA and chemiluminescence(CL)assay.Results:Serum IL-34 level in PBC patients was significantly higher than that in healthy controls(P<0.01).There was a correlation between level of IL-34 and AMA-M2.In patients who responded to treatment,serum IL-34 levels were significantly reduced.Serum levels of IL-2,IL-6 and TNF-αin PBC patients were significantly higher than those in healthy controls.Conclusion:Novel role of IL-34 in development of PBC and its potential use as a therapeutic target provide support.Serum IL-34 may be a candidate biomarker for PBC.