基于碳稳定同位素比值和矿物元素区分贵州不同产地的博落回花

Distinguish of Macleaya cordata(Willd.)R.Br.Flowers of Guizhou Province with Carbon Stable-Isotope Ratio and Mineral Elements Composition

探讨碳同位素比值和矿物元素在博落回花的产地推断中的可行性,为毒蜂蜜来源判别以及博落回原材料地理推断提供技术支撑。采集贵州省12个地域的博落回样本,采用元素分析-稳定同位素质谱仪(EA-IRMS)和电感耦合等离子体质谱仪(ICP-MS)测定博落回花的碳同位素比值(δ^(13)C)和19种矿物元素含量,结合主成分分析、偏最小二乘判别分析和正交偏最小二乘判别分析,建立了博落回花产地推断模型。根据矿物元素主成分分析,五个主成分代表了总指标88.289%的信息,Cs、Ba、Al、V、Mn、Fe、Ni和Li可做因子分析。基于正交-偏最小二乘法判别分析方法建立的判别模型确定了As、Rb、Pb、δ^(13)C、Cs、Cu、Sr共7个指标,可进一步建立博落回花的指纹图谱。基于碳同位素比值和矿物元素可用于区分博落回花的生产地域。

To explore the feasibility of carbon stable isotope ratio combined with mineral element in the identification of origin of Macleaya cordata(Willd.)R.Br.flowers,and provide technical support for distinguishing the geographical origin of poison honey and the traditional Chinese medicine.Samples of Macleaya cordata were collected from 12 regions of Guizhou,theδ^(13)C and 19 mineral element in flowers were determined by elementary analysis-isotope ratio mass spectrometers(EA-IRMS)and inductively coupled plasma mass spectrometry(ICP-MS).Combined with principle component analysis,partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis,a discriminant model of Macleaya cordata flowers producing area was established.With the analysis of mineral elements,the five principal components representing 88.289%of the total index,and Cs,Ba,Al,V,Mn,Fe,Ni and Li can be used for factor analysis.Based on the discriminant model established by orthogonal partial least squares discriminant analysis,the importance of 7 elements As、Rb、Pb、δ^(13)C、Cs、Cu、Sr were determined.Based on the differences ofδ13C and mineral element fingerprints,Macleaya cordata flowers from different habitats can be identified.