滞育时间对豆天蛾幼虫营养成分及代谢酶活性的影响

Effects of diapause time on nutritional components and metabolic enzymes activities in Clanis bilineata tsingtauica larvae

为了明确不同滞育时间对豆天蛾幼虫的影响,本研究测定了豆天蛾幼虫体内营养物质(包括蛋白质、氨基酸和脂肪酸)含量及肠道代谢酶活性的变化。结果表明,不同滞育时间的豆天蛾幼虫体内蛋白质含量均显著高于对照,且在滞育后期(150 d)达到最高值。滞育150 d的豆天蛾幼虫体内18种氨基酸含量均显著高于对照。与对照相比,滞育60 d豆天蛾幼虫体内亚油酸含量降低0.030个百分点(P<0.05),滞育30 d、90 d、120 d、150 d豆天蛾幼虫体内亚麻酸含量分别高于对照0.140个百分点、0.060个百分点、0.060个百分点、0.080个百分点(P<0.05)。滞育30 d豆天蛾幼虫肠道碱性磷酸酶、己糖激酶活性显著低于对照(P<0.05),滞育120 d时达到最高值;海藻糖酶、丙酮酸激酶活性在滞育90 d达到最高值;滞育120 d山梨醇脱氢酶活性最高,比对照高18.37%(P<0.05)。综上所述,滞育期的豆天蛾幼虫营养价值提高,代谢酶活性波动明显。

To clarify the effects of diapause time on Clanis bilineata tsingtauica larvae,the contents of nutrients(including protein,amino acids,and fatty acids)and the activities of intestinal metabolic enzymes in the larvae were measured.The results showed that the protein content in diapause larvae was significantly higher than that in control,and reached the highest value in the late diapause period(150 d).The contents of 18 amino acids at 150 days of diapause were significantly higher than those of the control.Compared with the control,the content of linoleic acid in the larvae of Clanis bilineata tsingtauica decreased by 0.030 percentage points(P<0.05)on the 60th day of diapause.At 30 d,90 d,120 d and 150 d of diapause,the content of linolenic acid in the larvae was 0.140 percentage points,0.060 percentage points,0.060 percentage points and 0.080 percentage points higher than that of the control(P<0.05).The activities of alkaline phosphatase and hexokinase in the intestine of the larvae at 30 days of diapause were significantly lower than those of the control,and reached the highest value at 120 days of diapause.The activities of trehalase and pyruvate kinase reached the highest value at 90 days of diapause.The activity of sorbitol dehydrogenase was the highest at 120 days of diapause,which was 18.37%higher than that of the control(P<0.05).In summary,the nutritional value of the diapause larvae was improved,and the activity of metabolic enzymes fluctuated significantly.