UHPLC-MS/MS测定不同产地市售南五味子药材中8种木脂素成分

Determination of Eight Lignans in Schisandrae Sphenantherae Fructus on the Market from Different Habitats by UPLC-MS/MS

目的比较12个产地市售南五味子中五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙、五味子酯甲、安五脂素、五味子酚的含量差异。方法样品用96孔蛋白沉淀微孔板进行前处理,采用ACE EXCEL 1.7 C18-AMIDE(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸水溶液(A)和甲醇(B)作为流动相进行梯度洗脱,流速为0.4 mL·min^(-1)。采用电喷雾离子源,正离子模式,多离子反应监测模式扫描。考察该方法的线性、仪器精密度、稳定性、重复性、加样回收率。结果12个产地中10个产地的五味子酯甲含量均达到中国药典2020年版对南五味子规定的≥0.2%的标准。结论UHPLC-MS/MS适宜南五味子中多成分的同时定量检测;市场所售南五味子品种基本符合国家法定标准。

OBJECTIVE To compare the contents of schizandrin A,schizandrin B,schizandrin C,schisandrol A,schisandrol B,schisantherin A,anwuligan,and schisanhenol in Schisandrae Sphenantherae Fructus on market from 12 habitats.METHODS The samples were pre-treated by 96-well fitration plates.The assay was performed on ACE EXCEL 1.7 C_(18)-AMIDE(100 mm×2.1 mm,1.7μm)column with 0.1%formic acid-water(A)and methanol(B),gradient elution,flow speed was 0.4 mL·min^(-1).Ion source was electric spray ion source,positive ion monitoring mode,multireaction monitoring mode for quantitative analysis.Linear,instrument precision,stability,repeatability,average recovery were investigated.RESULTS The content of schisantherin A in 10 of 12 producing areas reached the standard of≥0.2%of Schisandrae Sphenantherae Fructus in 2020 Edition of Chinese Pharmacopoeia.CONCLUSION UHPLC-MS/MS is suitable for simultaneous determination of multiple components in Schisandrae Sphenantherae Fructus.The Schisandrae Sphenantherae Fructus in the market basically meet the national legal standards.