基于HPLC指纹图谱与主成分分析相结合的四黄烧伤膏提取工艺研究

Study on extraction technology for Sihuang Shaoshang ointment based on HPLC fingerprint and principal component analysis

目的利用正交试验优选四黄烧伤膏的提取工艺,并建立四黄烧伤膏HPLC指纹图谱,为该制剂的质量评价提供参考。方法以四黄烧伤膏复方为模型药物,采用HPLC法建立指纹图谱,对指纹图谱共有峰峰面积进行主成分分析,以总因子得分为评价指标,采用正交试验法,对提取次数、提取时间、溶剂倍量等条件进行优化,筛选出最优提取工艺参数。结果在特征图谱研究中,共确定了12个共有峰。利用主成分分析法提取的2个主成分累计方差贡献率达到84.751%,计算9个样本的综合得分指标。正交试验得出的最佳提取工艺为加水10倍量,提取3次,每次2 h。结论该研究所建立的方法简单有效,适合四黄烧伤膏复方的提取,可以为四黄烧伤膏提取工艺优化提供参考。

Objective To optimize extraction technology for Sihuang Shaoshang ointment by orthogonal test,and establish the HPLC fingerprint of Sihuang Shaoshang ointment to provide references for the quality evaluation of the preparation.Methods Taking Sihuang Shaoshang ointment as the model drug,the fingerprints were established by HPLC method,the common peak areas were analyzed by principal component analysis,and the total factor scores were used as the evaluation index.The technological parameters such as extraction times,extraction time and liquid-solid ratio were optimized by orthogonal test,and the optimal extraction process parameters were screened out.Results The common mode of fingerprint was set up with 12 common peaks and the two principle components with the accumulative contribution rate of 84.751%extracted by principal component analysis were screened out to calculate the comprehensive scores of 9 samples.The optimal extraction process was as follows:10-fold amount of water,extracting 3 times,and extracting 2 h for each time.Conclusion The established method is simple and effective,which can provide a reference for the extraction process optimization of Sihuang Shaoshang ointment.