基于HPLC-QAMS多指标成分定量控制联合多元统计分析及加权TOPSIS法的食凉茶综合质量评价

Comprehensive quality evaluation of Shiliang tea based on HPLC-QAMS combined with multivariate statistical analysis and E-TOPSIS method

目的高效液相一测多评(HPLC-QAMS)法测定食凉茶中10种成分,并联合多元统计分析和加权优劣解距离(E-TOPSIS)法评价其综合质量。方法色谱柱为COSMOSIL5 C_(18)-MS-Ⅱ柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液,梯度洗脱,流速为1.0 mL·min^(-1),检测波长为360 nm和222 nm。以芦丁为参照,结合QAMS法,对18批食凉茶中东莨菪苷、东莨菪内酯、异嗪皮啶、芦丁、山柰酚-3-O-芸香糖苷、紫云英苷、滨蒿内酯、槲皮素、山柰酚和夏蜡梅碱的含量进行测定。运用统计软件对18批食凉茶的含量数据进行化学计量分析;用E-TOPSIS法对食凉茶质量进行综合评价。结果10个成分均实现良好分离,并在各自的范围内与峰面积呈良好的线性关系(r>0.9990);平均加样回收率为96.98%~100.12%,RSD<2.0%(n=9)。芦丁与其他9个成分的平均相对校正因子分别为2.1157、2.5924、0.5531、0.8976、0.7807、1.1593、0.6936、1.4583和0.6017,RSD<2.0%(n=6),QAMS法和常规外标法的定量结果显示两者无显著差异。多元统计分析结果显示,2个主成分的累计方差贡献率为83.886%,变量重要性投影值大于1的为山柰酚-3-O-芸香糖苷、芦丁和紫云英苷。E-TOPSIS分析结果显示,最优解的欧氏贴近度在0.1804~0.7394之间。结论建立的方法简便、准确且经济实用,可全面反映食凉茶的质量差异性,能较好地控制该品种质量。

Objective To determine 10 components in Shiliang tea by high performance liquid chromatography-quantitative analysis of multi-components with a single-marker(HPLC-QAMS),and to evaluate its comprehensive quality by multivariate statistical analysis and entropy-technique for order preference by similarity to ideal solution(E-TOPSIS).Methods The determination was performed on COSMOSIL®5 C_(18)-MS-Ⅱcolumn(250 mm×4.6 mm,5μm)with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution and gradient elution at the flow rate of 1.0 mL·min^(-1),and the detection wavelength was set at 360 nm and 222 nm.The contents of scopolin,scopoletin,isofraxidin,rutoside,kaempferol-3-O-rutinoside,astragalin,scoparone,quercetin,kaempferol and mcalycanthine in 18 batches of Shiliang tea were calculated according to QAMS using rutoside as internal reference.The content data of 18 batches of Shiliang tea were analyzed chemometrically using statistical software;the quality of Shiliang tea was evaluated comprehensively using E-TOPSIS method.Results Good separation was obtained for all 10 components and showed good linearity with peak area in their respective scopes(r>0.9990).The average recovery rates were 96.98%-100.12%(RSD<2.0%,n=9).The average relative correction factors for rutoside and the other 9 components were 2.1157,2.5924,0.5531,0.8976,0.7807,1.1593,0.6936,1.4583 and 0.6017(RSD<2.0%,n=6),and there was no significant difference between the contents obtained by QAMS and external standard method for each component.The results of multivariate statistical analysis showed that the cumulative variance contribution rate of the 2 principal components was 83.886%,and the variable importance in the projection value greater than 1 were kaempferol-3-O-rutinoside,rutoside and astragalin.The results of E-TOPSIS showed that the euclidean closeness of the optimal solution was between 0.1804 and 0.7394.Conclusion The method established is simple,accurate,economical and practical,which can fully reflect the quality difference of Shiliang tea and better control the quality of this variety.