HPLC多指标成分定量、化学计量学及熵权-TOPSIS分析在龙葵综合质量评价中的应用

Application of HPLC multi-components quantification combined with chemometrics and entropy weight-TOPSIS method in comprehensive quality evaluation of Solanum nigrum

目的建立不同产地龙葵中11个成分含量同步检测方法,并采用化学计量学和熵权-TOPSIS法对其质量进行评价。方法收集8省17个批次龙葵样品,采用HPLC法同时检测龙葵中梣皮树脂醇、松脂素、槲皮素、芦丁、澳洲茄碱、澳洲茄边碱、客西茄碱、澳洲茄胺、去半乳糖替告皂苷、薯蓣皂苷元和β-谷甾醇的含量,建立龙葵多组分定量控制模式;采用化学识别模式和熵权-TOPSIS法建立龙葵质量优劣评价模型,对其整体质量进行综合评价。结果11个成分分别在0.78~39.00,0.55~27.50,0.34~17.00,0.21~10.50,41.87~2093.50,60.95~3047.50,2.58~129.00,1.02~51.00,0.46~23.00,1.05~52.50,0.42~21.00μg/mL(r>0.9990)范围内线性关系良好,平均加样回收率在96.81%~100.28%范围内(RSD<2.0%,n=9);17批样品聚为3类;澳洲茄边碱、澳洲茄碱、去半乳糖替告皂苷和梣皮树醇可能是影响龙葵产品质量主要潜在标志物;熵权-TOPSIS法分析结果显示17批龙葵质量评价贴近度分别为0.4336、0.4168、0.6242、0.5008、0.4791、0.6361、0.5683、0.2500、0.1909、0.2221、0.1707、0.7200、0.6983、0.7447、0.7179、0.7209、0.7183,辽宁、吉林和黑龙江产地龙葵整体质量较好,其次为江苏、河南和安徽产地。结论建立的同时测定龙葵中11种成分含量的HPLC法,操作便捷,结果准确;化学计量学及熵权-TOPSIS法客观全面,可用于龙葵的整体质量评价。

Objective To establish a method for simultaneous determination of 11 components of Solanum nigrum from different producing areas,and to evaluate the quality by chemometrics and entropy weight-technique for order preference by similarity to ideal solution(EW-TOPSIS).Methods The 17 batches of Solanum nigrum samples from 8 provinces were collected.The high performance liquid chromatography(HPLC)method was used to simultaneously determine the contents of medioresino,pinoresinol,quercetin,rutoside,solasonine,solamargine,khasianine,solasodine,desgalactotigonin,diosgenin andβ-sitosterol,and the multi-components quantitative control mode of Solanum nigrum was established.The quality evaluation model of Solanum nigrum was established by using chemical recognition pattern and EW-TOPSIS method,and the overall quality was evaluated comprehensively.Results When the 11 components were in the 0.78-39.00,0.55-27.50,0.34-17.00,0.21-10.50,41.87-2093.50,60.95-3047.50,2.58-129.00,1.02-51.00,0.46-23.00,1.05-52.50 and 0.42-21.00μg/mL(r>0.9990),their linear relationships were good.The average recovery was 96.81%-100.28%with the RSD<2.0%(n=9).17 batches of samples clustered into 3 categories.Solamargine,solasonine,desgalactotigonin and medioresino may be the main potential markers affecting the quality of Solanum nigrum.The results of EW-TOPSIS method showed that,the quality evaluation closeness of 17 batches of Solanum nigrum were 0.4336,0.4168,0.6242,0.5008,0.4791,0.6361,0.5683,0.2500,0.1909,0.2221,0.1707,0.7200,0.6983,0.7447,0.7179,0.7209 and 0.7183,respectively,indicating that the overall quality of Solanum nigrum from Liaoning,Jilin and Heilongjiang were better,followed by Jiangsu,Henan and Anhui.Conclusion The established HPLC method for simultaneous determination of 11 components in Solanum nigrum is convenient and accurate.Chemometrics and EW-TOPSIS method are objective and comprehensive,which can be used for the overall quality evaluation of Solanum nigrum.