丙泊酚对慢性睡眠剥夺社交行为障碍大鼠内侧前额叶皮层小清蛋白神经元的影响

Effect of propofol on parvalbumin neurons in medical prefrontal cortex of rats with social behavior disorders induced by chronic sleep deprivation

目的评价丙泊酚对慢性睡眠剥夺社交障碍大鼠内侧前额叶皮层(mPFC)小清蛋白(PV)神经元的影响。方法SPF级健康雄性SD大鼠42只,8周龄,体质量200~250 g,采用随机数字表法分为3组(n=14):对照组(Con组)、慢性睡眠剥夺+自然睡眠组(CSD+NS组)和慢性睡眠剥夺+丙泊酚组(CSD+Pro组)。采用改良多平台水环境法建立大鼠睡眠剥夺模型,每天睡眠剥夺20 h,自然睡眠4 h,连续28 d。CSD+Pro组于睡眠剥夺后腹腔注射丙泊酚40 mg/kg,连续28 d。Con组和CSD+NS组腹腔注射等容量10%脂肪乳剂。睡眠剥夺结束后采用三箱社交实验检测大鼠社交行为,采用免疫荧光法检测mPFC区PV阳性神经元数目和神经元周围网络(PNN)密度。结果与Con组比较,CSD+NS组快眼动睡眠百分比、三箱社交实验中嗅探时间偏好系数1和嗅探时间偏好系数2降低,mPFC区PV阳性神经元计数和PNN密度减少(P<0.05);与CSD+NS组比较,CSD+Pro组三箱社交实验中嗅探时间偏好系数1和嗅探时间偏好系数2增加,mPFC区PV阳性神经元计数和PNN密度增加(P<0.05),快眼动睡眠百分比差异无统计学意义(P>0.05)。结论丙泊酚可能增加PV神经元数量和功能,改善睡眠剥夺大鼠社交行为障碍。

Objective To evaluate the effect of propofol on parvalbumin(PV)neurons in the medical prefrontal cortex(mPFC)of rats with social behavior disorders induced by chronic sleep deprivation.Methods Forty-two SPF male Sprague-Dawley rats,aged 8 weeks,weighing 200-250 g,were divided into 3 groups(n=14 each)using a random number table method:control group(group Con),chronic sleep deprivation plus natural sleep group(group CSD+NS),and chronic sleep deprivation plus propofol group(group CSD+Pro).Sleep deprivation model was established by the modified multiple platform method,the rats were placed in the sleep-deprivation tank for 20 h a day(14:00-10:00),and allowed to sleep naturally for 4 h(10:00-14:00)a day for 28 consecutive days.Propofol 40 mg/kg was intraperitoneally injected for 28 consecutive days after sleep deprivation in CSD+Pro group.While the equal volume of 10%fat emulsion was given in Con and CSD+NS groups.After the end of sleep deprivation,a three-box social experiment was used to detect the social behavior of rats,and the number of the PV positive cells and density of the perineuronal network(PNN)in the mPFC area were measured by immunofluorescence.Results Compared with group Con,the pertentage of rapid eye movement sleep and sniffing time preference coefficients for the strange rat 1 in the first stage and for the strange rat 2 in the second stage were significantly decreased,and the number of the PV positive cells and density of PNN in the mPFC area were decreased in group CSD+NS(P<0.05).Compared with group CSD+NS,the sniffing time preference coefficients for the strange rat 1 in the first stage and for the strange rat 2 in the second stage were significantly increased,the number of the PV positive cells and density of PNN in the mPFC area were increased(P<0.05),and no significant change was found in the percentage of the rapid eye movement sleep in group CSD+Pro.Conclusions Propofol probably increases the number and function of PV neurons in the mPFC and ameliorates sleep deprivation-induced social behavior disorders in sleep-deprived rats.