玉米秸秆降解复合菌系的筛选及发酵产短链脂肪酸

Screening of maize stover degradation complex strains and fermentation to produce short�chain fatty acids

利用筛选得到的复合菌系来发酵玉米秸秆,研究复合菌系对降解秸秆产短链脂肪酸的影响,并对微生物菌群结构组成进行解析。结果表明,复合菌系在前4 d的降解速度最快,秸秆、纤维素和木质素的降解率分别达30.35%、27.07%和14.71%,在12 d时,其降解率分别达40.02%、32.25%和20.93%。复合菌系的代谢产物以异丁酸和乙酸为主,其含量分别占总短链脂肪酸的36.46%和33.15%。由高通量测序可知,复合菌系中的优势菌属主要由Clostridium sensu stricto、Cellulosilyticum、Clostridium XIVa、Enterobacteriaceae和Peptostreptococcaceae等组成。

The compound strains obtained by screening were used to ferment corn straw,the effects of the compound strains on degrading straw to produce short-chain fatty acids were studied,and the structural composition of the microbial consortium was analyzed.The results showed that the degradation rate of the compound strain was the fastest in the first 4 days.The degradation rates of straw,cellulose and lignin reached 30.35%,27.07%and 14.71%,respectively.At 12 days,the degradation rates reached 40.02%,32.25%and 20.93%,respectively.The metabolites of the compound strain were isobutyric acid and acetic acid,which accounted for 36.46%and 33.15%of the total short chain fatty acids,respectively.According to high-throughput sequencing,the dominant bacteria genera in the compound strains were mainly composed of Clostridium sensu stricto,Cellulosilyticum,Clostridium XIVa,Enterobacteriaceae and Peptostreptococcaceae.