核桃青皮不同溶剂提取物抗氧化和抑菌活性对比研究

A Comparative Study on the Antioxidant and Antibacterial Activities of Different Solvent Extracts from Walnut Green Husk

为探究核桃青皮潜在的功能活性,以90%乙醇、石油醚、乙酸乙酯、正丁醇和蒸馏水为溶剂,采用系统溶剂法对核桃青皮活性物质进行了提取,并对不同溶剂萃取物进行了体外抗氧化能力和抑菌活性测定。结果表明:90%乙醇对核桃青皮的提取率为36.79%,总酚和胡桃醌提取量分别为70.36、1.38 mg·g^(-1)。乙酸乙酯萃取物对·OH和O_(2)^(-)·清除率分别为37.52%、56.53%,高于其他溶剂萃取物。在抑菌活性方面,不同溶剂萃取物对5种供试菌表现出不同的抑菌效果,正丁醇萃取物>90%乙醇提取物>乙酸乙酯萃取物>萃余水萃取物>石油醚萃取物。综上,核桃青皮提取物表现出良好的潜在抗氧化和抗菌活性,具有开发植物源抗氧化剂和抑菌剂的潜力。

In order to explore the potential functional activity of walnut green husk,this study used 90%ethanol,petroleum ether,ethyl acetate,n-butanol and distilled water as solvents to extract the active substances of walnut green husk by system solvent method,and the in vitro antioxidant capacity and bacteriostatic activity of the different solvent extracts were determined.The results showed that the extraction rate of walnut green husk by 90%ethanol was 36.79%,and the extraction amounts of total phenol and juglone were 70.36 mg·g^(-1)and 1.38 mg·g^(-1),respectively.The clearance rates of·OH and O_(2)^(-)·of ethyl acetate extract were 37.52%and 56.53%,which were higher than those of other solvent extracts.In terms of antibacterial activity,different solvent extracts showed different antibacterial effects on 5 tested bacteria,n-butanol extract>90%ethanol extract>ethyl acetate extract>raffinate water extract>petroleum ether extract.In conclusion,walnut green husk extract showes good potential antioxidant and antibacterial activities,and has the potential to develop plant-derived antioxidants and antibacterial agents.