新型缓释载体材料在草地贪夜蛾引诱剂中的应用

Application of new dispenser material in the lure of Spodoptera frugiperda

比较不同草地贪夜蛾引诱剂的田间诱集效果差异,筛选出长持效期引诱剂使用的缓释载体材料,为草地贪夜蛾的长期种群监测提供重要的理论依据。本研究首先将新型材料海藻酸钙/聚己内酯微球作为缓释载体应用于草地贪夜蛾引诱剂1,对其结构性能进行相关分析;通过田间诱集试验比较引诱剂1、引诱剂2、引诱剂3、引诱剂4、引诱剂5等五种不同草地贪夜蛾引诱剂的田间持续监测诱集效果;利用气相色谱仪定量比较两种诱集效果不同载体材料的引诱剂1(最优)、引诱剂2(最差)中所含性信息素组分的缓释能力。结果表明:新型材料海藻酸钙/聚己内酯微球的蛙卵状球形结可使草地贪夜蛾引诱剂1中信息素组分缓慢释放,室内静置2个月后该载体基本结构保持稳定;五种不同草地贪夜蛾引诱剂的持续诱捕能力对比为:引诱剂1>引诱剂5>引诱剂4>引诱剂3>引诱剂2,引诱剂1使用80 d仍然具有很强的引诱效果;通过对比诱集持续效果最优及最差的2种引诱剂1、引诱剂2所用载体的缓释性能,发现田间诱集持续效果最好的引诱剂1对草地贪夜蛾性信息素组分的缓释效果显著优于引诱剂2。海藻酸钙/聚己内酯微球作为载体材料可使草地贪夜蛾引诱剂的诱集持效期达80 d以上,可实现对草地贪夜蛾危害周期全覆盖,为持续开展草地贪夜蛾种群监测奠定了重要理论基础。

To provide an important theoretical basis for the long-term population monitoring of Spodoptera frugiperda,we compare the field attraction effects of different lures on S.frugiperda,and select the dispenser material for the best attractant with prolonged release duration.In this study,SA@Ca 2+/PCL microsphere was used as lure dispenser material for S.frugiperda.The attraction effects of five different lure compounds in filed monitoring assays were compared.Besides,the release dynamics of pheromone components in lure 1 and lure 2,which were carried with two different dispenser materials were compared by gas chromatography.The results supported that SA@Ca 2+/PCL microsphere enabled to release the pheromone component slowly,and the basic structure of the dispenser material remained stable after 2 months in the laboratory.The continuous trapping ability of five different lures were ranked as follows:lure 1>lure 5>lure 4>lure 3>lure 2.It was worthy to note that lure 1 kept strong attraction effect after 80 d in the field.By comparing the sustained-release properties of lure 1 and lure 2 with different attractant effects,it was found that the sustained-release effect of lure 1 with long duration on the pheromone components in field attractant was significantly better than that of lure 2.SA@Ca 2+/PCL microsphere has strong dispenser capacity over 80 d,which cover the full damage cycle of S.frugiperda,and this lays an important foundation for the continuous monitoring population of S.frugiperda.