望谟紫芽茶树优良品系‘W016’与黄金芽抗茶蚜性能比较

Comparison of resistance of Wangmo Purple bud tea‘W016’and‘Huangjinya’to Toxoptera aurantii(Hemiptera:Aphididae)

茶蚜Toxoptera aurantii Boyer是茶树上的重要害虫之一,深入研究抗蚜特异资源的抗性机理对培育茶树抗蚜品种具有重要意义。课题组前期收集的望谟紫芽茶树优良品系‘W016’对茶蚜抗性强,为初步探明该种质的抗蚜特性,利用刺吸电位技术测定了茶蚜在‘W016’和黄金芽(感虫品种)上的取食行为,并探明影响茶蚜刺探取食叶片的主要物理性状及生化成分因子。结果表明,茶蚜在两种供试种质上均产生9种EPG波形,依次为非刺探波(np波)、路径波(A波、B波、C波、pd波)、机械阻力波(F波)、木质部吸食波(G波)、韧皮部唾液分泌波(El波)和韧皮部汁液吸食波(E2波)。刺探取食过程中,茶蚜在‘W016’上的非刺探时间(np波)和C波总持续时间均显著长于黄金芽。二者口针遇到阻力的次数(F波)和总持续时间无差异,但韧皮部吸食汁液(E2波)时间‘W016’显著短于黄金芽。茸毛密度、下表皮厚度和栅栏组织厚度是‘W016’抗茶蚜取食胁迫的主要物理屏障因子,而咖啡碱和可溶性糖含量为主要生化因子。表明‘W016’对茶蚜的取食抗性与其叶片理化性质密切相关。

The tea aphid,Toxoptera aurantii Boyer(Hemiptera:Aphididae),is one of the important pests on tea plants.Lucubrating the resistance mechanism of specific T.aurantia resistant resources is crucial significance for breeding resistant tea varieties.The Wangmo excellent purple bud tea germplasm‘W016’by our research group in the early stage has strong resistance to T.aurantii.Here,to preliminarily explore the resistance characteristics of this germplasm to T.aurantii.,the feeding behavior of T.aurantii on‘W016’and‘Huangjinya’(susceptible cultivar)was measured using electrical penetration graph technology,and the main physical characteristics and nutritional component factors affecting the probing and feeding activities of T.aurantia were identified on leaves.The results showed that T.aurantii produced 9 EPG waveforms on two tea germplasms,including non-probing behavior(np),intercellular stylet pathway(A,B,C,and pd),derailed stylet mechanics(F),xylem sap ingestion(G),phloem salivation(El)and passive phloem ingestion(E2).During the process of probing and feeding,the sum time of np and total duration of C waves of T.aurantia on‘W016’were significantly longer than that on‘Huangjinya’.No significant differences were observed in the number of F and duration of F between the two tested germplasms.However,the duration of E2 of T.aurantii on‘W016’was significantly shorter than on‘Huangjinya’.Additionally,trichome density,lower epidermis thickness and palisade tissue thickness were the main physical barriers factors of‘W016’resistant to T.aurantii feeding stress,and contents of caffeine and soluble sugar were the main nutritional factors.The results indicated that the feeding resistance of‘W016’to T.aurantii was closely related to the physicochemical properties of its leaves.