月季花标准汤剂UPLC指纹图谱及多指标成分定量研究

Fingerprint and multi-components content determination of standard decoction of Rosae Chinensis Flos

建立月季花(Rosae Chinensis Flos)标准汤剂UPLC指纹图谱,为月季花标准汤剂质量控制提供参考。通过超高效液相色谱法(UPLC)建立20批月季花标准汤剂指纹图谱,共有色谱峰通过质谱分析和对照品比对进行指认,采用相似度评价结合化学模式识别方法探究影响月季花标准汤剂质量的特征性成分,并同时测定主要色谱峰中的8个成分的含量。结果建立了月季花标准汤剂的UPLC指纹图谱,并标定出15个共有峰,20批样品相似度在0.983~0.999;聚类分析(HCA)和主成分分析(PCA)将样品分为4类,结合偏最小二乘法判别分析(OPLS-DA)筛选出11个标志性化合物,通过质谱鉴定和对照品确认了8个成分;含量测定结果显示,河南省周口市的月季花标准汤剂没食子酸、鞣花酸、异槲皮苷、紫云英苷和槲皮素含量显著高于其他产地的样品。研究建立的月季花标准汤剂分析方法稳定可行,方法重现性好,能够为月季花标准汤相关制剂开发、质量标准研究提供参考。

To establish an UPLC method for the fingerprint analysis and content determination of standard decoction of Rosae Chinensis Flos(RCF),and to provide reference for quality control of standard decoction of RCF,the fingerprints of 20 batches of standard decoction of RCF was established by ultra-high performance liquid chromatography(UPLC),and the common fingerprint peaks were identified by mass spectrometry analysis and comparison with reference materials.The similarity evaluation combined with chemical pattern recognition were applied to explore characteristic components affecting the quality of standard decoction of RCF,and the content of eight components in the main fingerprint peaks were determined simultaneously.Results the UPLC fingerprint of standard decoction of RCF was established,and 15 fingerprint peaks were identified,and the similarity of 20 batches of samples was 0.983-0.999.Hierarchical cluster analysis(HCA)and principal analysis(PCA)divided the samples into four categories,and combined with partial leats square discriminant analysis(OPLS-DA)to screened out 11 signature compounds,of which eight components were confirmed by mass spectrometry and control products.The results showed that the content of gallic acid,ellagic acid,isoquercetin,astragalin and quercetin in the standard decoction of RCF from Zhoukou,Henan Province were significantly higher than those from other areas.The analysis method established of standard decoction of RCF is stable,feasible and reproducible,which can provide a reference for the relative preparation development and quality standard research of standard decoction of RCF.