ApoE基因多态性与冠心病患者呼吸道感染的关联

Association of Apo E gene polymorphisms with respiratory tract infection in patients with coronary heart disease

目的 探究载脂蛋白E(ApoE)基因多态性与冠心病患者呼吸道感染的关联.方法 选取衡水市人民医院于2021年1月-2023年3月收治的125例冠心病患者为研究对象,根据呼吸道感染情况分为感染组48例、非感染组77例;分析呼吸道感染病原菌情况,采用聚合酶链式反应(PCR)检测ApoE基因多态性,比较两组患者ApoE基因多态性,采用Logistic回归分析法归纳冠心病患者呼吸道感染的危险因素.结果 48例感染患者共培养分离病原菌62株,其中革兰阳性菌22株,革兰阴性菌38株,真菌2株,以大肠埃希菌、肺炎克雷伯菌和肺炎链球菌为主;ApoE基因存在3种等位基因e2、e3、e4,共有6种基因型e2/2、e3/3、e4/4、e2/3、e2/4、e3/4,感染组的e3/3基因型及e3等位基因频率低于非感染组,而e4/4基因型及e4等位基因频率高于非感染组(均P<0.05);ApoE e4携带者是冠心病患者呼吸道感染的危险因素,而ApoE e3携带者是冠心病患者呼吸道感染的保护因素(P<0.05).结论 ApoE基因多态性与冠心病患者呼吸道感染存在相关性,其中e4等位基因可能促进呼吸道感染,而e3等位基因则抑制呼吸道感染.

OBJECTIVE To investigate the association of apolipoprotein E(Apo E)gene polymorphisms with respir-atory tract infections in patients with coronary heart disease.METHODS Totally 125 cases of patients with coro-nary heart disease admitted to Hengshui People's Hospital from Jan.2021 to Mar.2023 were selected as the study subjects,and they were divided into an infection group of 48 cases and a non-infection group of 77 cases according to respiratory tract infections.The distribution of respiratory infectious pathogens were analyzed,Apo E gene pol-ymorphisms were detected by polymerase chain reaction(PCR),the distribution characteristics of ApoE gene pol-ymorphism between the two groups of patients were compared,and logistic regression analysis was used to sum-marize the risk factors affecting respiratory tract infections in patients with coronary heart disease.RESULTS A to-tal of 62 strains of pathogenic bacteria were cultured and isolated from 48 infected patients,including 22 strains of gram-positive bacteria,38 strains of gram-negative bacteria,and 2 strains of fungi,with Escherichia coli,Kleb-siella pneumoniae,and Streptococcus pneumoniae predominating.There were three alleles of Apo E gene,e2,e3,and e4,with a total of six genotypes:e2/2,e3/3,e4/4,e2/3,e2/4,and e3/4.The genotype e3/3 and the allele frequency of e3 in the infection group were lower than those in the non-infection group,while the genotype e4/4 and the allele frequency of ε4 were higher than those in the non-infection group(all P<0.05).Apo E e4 allele car-riers was a risk factor for respiratory tract infections in patients with coronary heart disease,while carrying the Apo E ε3 allele carriers were a protective factor for respiratory tract infections in patients with coronary heart dis-ease(P<0.05).CONCLUSION Apo E gene polymorphisms were associated with respiratory tract infections in pa-tients with coronary heart disease,in which the e4 allele might promote respiratory tract infections,while e3 allele might inhibit respiratory tract infections.