光谱法研究天然牛磺酸与肝硬化门静脉高压大鼠的肝组织蛋白相互作用

Study on the interaction of taurine and hepatic protein in rats with cirrhotic portal hypertension by spectrometry

目的:通过光谱法研究天然牛磺酸(Taurine)与肝硬化门静脉高压大鼠的肝组织蛋白相互作用并探讨其作用机制。方法:将120只大鼠按随机数字法分成正常组、模型组、心得安组、天然牛磺酸组四组,每组30只,除正常组外,其余组用四氯化碳(carbon tetrachloride, CCL4)制备肝硬化门脉高压大鼠模型,造模同时给药。心得安组喂心得安,天然牛磺酸组喂天然牛磺酸,模型组喂等量生理盐水,疗程10周,治疗后对比各组门脉高压数值,并测定不同温度下各组天然牛磺酸药物-大鼠肝组织蛋白体系荧光光谱和天然牛磺酸紫外吸收光谱,根据光谱数据进行分析。结果:天然牛磺酸组大鼠门脉血流量(portal vein flow, PVF)、门脉压力(portal vein pressure, PVP)、门脉阻力(PVR)较心得安组、模型组更低,平均动脉压(mean arterial pressure, MAP)较高。各组大鼠肝组织蛋白的Kq值均大于最大动态猝灭速率2.0×10^(10)L/(mol·s)。大鼠肝组织蛋白体系:焓变(△H):-8 707.633 6;熵变(△S):33.413 95。正常组吉布斯自由能变化(△G)、△H均为负值,△S为正值;天然牛磺酸组△G、△H均为正值,△S为负值,模型组无明显规律。正常组、模型组、天然牛磺酸组R值分别为1.77、2.35、2.28 nm, r值分别为1.24、1.31、6.56。结论:天然牛磺酸具有抗肝纤维化,减轻门脉高压的作用,其机制可能是改变了肝硬化门脉高压大鼠的肝脏组织蛋白结构,从而减轻肝损害。

Objective:To study the interaction of taurine with hepatic proteins in rats with cirrhotic portal hypertension and its underlying mechanism using spectroscopy.Methods:A total of 120 rats were randomly divided into the normal group,model group,propranolol group and taurine group,with 30 rats in each group.Except for the normal group,the other three groups were treated with carbon tetrachloride(CCL4)to prepare a cirrhotic portal hypertension rat model.When modeling,the propranolol group was given propranolol,the taurine group was treated with taurine,and rats in the model group were fed with the same amount of normal saline.All rats were treated for a course of 10 weeks.After treatment,values of portal hypertension in each group were compared.The fluorescence spectra of hepatic protein and the ultraviolet absorption spectrum of taurine of rats in each group at different temperatures were measured and analyzed.Results:Compared to the propranolol group and model group,portal venous flow(PVF),portal venous pressure(PVP),portal vascular resistance(PVR)and heart rate were lower and mean arterial pressure(MAP)was higher in the taurine group.Kq values of hepatic protein for each rats were greater than the maximum dynamic quenching rate of 2.0×10^(10)L/(mol·s),with the enthalpy change(△H)of-8707.6336 and entropy change(△S)of 33.41395.△G and△H of Gibbs free energy change in the normal group were both negative,and△S was positive.△G and△H in the taurine group were positive,with negative△S,while the model group had no obvious regularity.The R values were 1.77,2.35,and 2.28 nm in the normal,model and taurine group respectively,with the r values of 1.24,1.31 and 6.56 respectively.Conclusion:Taurine can inhibit fibrosis and attenuate portal hypertension,and this mechanism may alter the protein structure of liver tissue in cirrhotic portal hypertension rats to alleviate liver damage.