艾纳香特选种质组培特性及克隆后代L-龙脑含量比较研究

Characteristics of tissue culture and L-borneol content in cloned progeny of selected Blumea balsamifera germplasm

目的建立高效的艾纳香Blumea balsamifera特选种苗克隆技术体系并获得高质量的候选种质。方法特选了通过初筛的3种L-龙脑不同含量(低、中、高)的种质母株,优化组培培养基配方,再比较母株和克隆苗中L-龙脑的含量。结果确定了最佳培养基配方,GC-MS分析证实,克隆苗中L-龙脑的含量与母株呈一致趋势。结论成功地建立了一种保持遗传稳定性的高效组织培养技术。添加适量的胺鲜酯(diethyl aminoethyl hexanoate)可显著提高再生苗的生根效率。研究还表明,所选艾纳香种质(H)中的高L-龙脑含量是由遗传因素决定的,并且高L-龙脑的特性保留在克隆苗的后代中。为艾纳香无性繁殖选育优良种苗提供了宝贵的优质候选种质,具有较好推广前景。

Objective To establish an efficient cloning technology system for Blumea balsamifera(L.)DC.seedlings and obtain high-quality candidate germplasm.Methods In this study,three maternal plants with varying levels of L-borneol content(low,medium,and high)were selected through preliminary screening.Tissue culture was conducted using different media,and the effects of each medium were compared.The L-borneol contents in the cloned seedlings and maternal plants were also compared.Results The optimal culture medium formula was determined,and GC-MS analysis confirmed that the L-borneol contents in the cloned offspring exhibited a consistent trend with the mother plants.Conclusion An efficient tissue culture technique that maintains genetic stability was successfu lly established in this study.The rooting efficiency of regenerated seedlings was significantly improved by adding an appropriate amount of diethyl aminoethyl hexanoate(DA-6).The study also identified that the high L-borneol content in the selected B.balsamifera germplasm(H)is determined by genetic factors,and the characteristic of high L-borneol is retained in the offspring of cloned plants.These results provide valuable high-quality candidate germplasms and serve as technical references for select ing superior B.balsamifera seedlings through asexual reproduction,with promising prospects for further promotion.