地榆皂苷Ⅰ调控IGFL2-AS1/miR-138-5p抑制卵巢癌细胞增殖、侵袭和迁移

Ziyuglycoside Ⅰ inhibits proliferation,invasion and migration of ovarian cancer cells by regulating IGFL2-AS1/miR-138-5p

目的 探讨地榆皂苷Ⅰ对卵巢癌细胞增殖、侵袭和迁移的影响及分子机制。方法 人卵巢癌A2780细胞分别用7.5、15.0、30.0μmol/L的地榆皂苷Ⅰ处理,同时设置si-NC组、si-IGFL2-AS1组、地榆皂苷Ⅰ+pc DNA-IGFL2-AS1组、地榆皂苷Ⅰ+pcDNA组;MTT检测细胞增殖抑制率;克隆形成实验检测细胞克隆形成数;Transwell法检测迁移和侵袭细胞数;Western blotting法检测E-钙黏蛋白(E-cadherin)和N-钙黏蛋白(N-cadherin)表达;双荧光素酶报告实验检测IGFL2-AS1和miR-138-5p的靶向关系。结果 不同浓度的地榆皂苷Ⅰ处理A2780细胞后,细胞增殖、迁移和侵袭能力显著减弱(P<0.05),Ecadherin和miR-138-5p表达上调(P<0.05),N-cadherin和IGFL2-AS1表达下调(P<0.05),呈剂量相关性。下调IGFL2-AS1抑制A2780增殖、迁移及侵袭(P<0.05)。IGFL2-AS1靶向调控miR-138-5p,过表达IGFL2-AS1可减弱地榆皂苷Ⅰ对A2780细胞增殖、迁移和侵袭的作用(P<0.05)。结论 地榆皂苷Ⅰ通过调控IGFL2-AS1/miR-138-5p抑制卵巢癌细胞增殖、侵袭和迁移。

Objective To explore the effect and molecular mechanism of ziyuglycoside Ⅰ on proliferation,invasion and migration of ovarian cancer cells.Methods Ovarian cancer cells A2780 were treated with 7.5,15.0,30.0μmol/L of ziyuglycoside Ⅰ.At the same time,si-NC group,si-IGFL2-AS1 group,ziyuglycoside Ⅰ+pcDNA-IGFL2-AS1 group and ziyuglycoside I+pcDNA group were set up.MTT was used to detect proliferation inhibition rate of cells;Clone formation experiment was used to detect cell clone formation number;Transwell method was used to detect the numbers of migrating and invading cells;Western blotting was used to detect Ecadherin and N-cadherin protein expressions;Dual luciferase reporter experiment was used to detect the targeting relationship between IGFL2-AS1 and miR-138-5p.Results After treatment of A2780 cells with different concentrations of ziyuglycoside Ⅰ,cell proliferation,migration and invasion were significantly weakened(P<0.05),expressions of E-cadherin and miR-138-5p were upregulated(P<0.05),and the expressions of N-cadherin and IGFL2-AS1 were down-regulated in a dose-dependent manner(P<0.05).After down-regulating IGFL2-AS1,the proliferation,migration and invasion of A2780 cells were inhibited(P<0.05).IGFL2-AS1 targeted and regulated miR-138-5p.Overexpression of IGFL2-AS1 could attenuate the effects of ziyuglycoside I on proliferation,migration and invasion of A2780 cells(P<0.05).Conclusion Ziyuglycoside I inhibits the proliferation,invasion and migration of ovarian cancer cells by regulating IGFL2-AS1/miR-138-5p.