珠子参对慢性咽炎模型大鼠的干预作用及其急性毒性研究

Study on Acute Toxicology and Intervention Effect of Panacis Majoris Rhizoma on Chronic Pharyngitis Rat Model

目的探究珠子参对大鼠慢性咽炎的干预作用及急性毒性。方法单次最大体积灌胃昆明种小鼠74.4 g·kg^(-1)珠子参,通过检测小鼠存活状态、脏器系数、主要脏器组织形态、血常规和生化指标来评价珠子参毒性。SD大鼠随机分为对照组、模型组、阳性对照(醋酸泼尼松片)组(6.25 mg·kg^(-1)),珠子参低、中、高剂量组(0.58,1.16,2.32 g·kg^(-1)),连续灌胃30 d,每天1次;除对照组外,其余各组采用乙型溶血性链球菌诱导大鼠感染慢性咽炎;处死大鼠后,测定血清炎症因子白细胞介素6(Interlukin-6,IL-6)、环氧合酶2(Cyclooxygenase-2,COX-2)、白细胞介素1β(Interlukin-1β,IL-1β)、细胞间黏附分子1(Intercellular celladhesion molecule-1,ICAM-1)、C反应蛋白(C-reactive protein,CRP)、肿瘤坏死因子α(Tumor necrosis factor,TNF-α)、单核细胞趋化蛋白1(Monocyte chemoattractant protein-1,MCP-1)、前列腺素E2(Prostaglandin E2,PGE2)水平,HE染色观察咽部组织形态改变。结果毒理研究发现,给予珠子参后,除小鼠血浆丙氨酸转氨酶(Alanine transaminase,ALT)水平(P<0.05)及脾脏系数(P<0.01)明显升高并伴随组织病理改变外,其余脏器未见明显病理变化,且血常规和血浆生化指标无明显变化;药效研究发现,珠子参明显降低慢性咽炎模型大鼠血清炎症因子水平(P<0.05,P<0.01,P<0.001),改善其咽部组织病理变化。结论珠子参可通过抑制炎症反应缓解乙型溶血性链球菌诱导的慢性咽炎,但对脾脏可能具有潜在毒性。

Objective To investigate the acute toxicology and intervention effect of Panacis Majoris Rhizoma on rats with chronic pharyngitis.Methods A single,maximum dose of Panacis Majoris Rhizoma(74.4 g·kg^(-1))was administered to Kunming mice to evaluate its toxicity,involving the assessment of the survival status of the mice,organ indices,morphological changes in major organs,blood routine,and biochemical indicators.SD rats were randomly divided into the control group,model group,prednisone group(6.25 mg·kg^(-1)),and low-,medium-,and high-dose Panacis Majoris Rhizoma groups(0.58,1.16,and 2.32 g·kg^(-1)).All rats received the corresponding drugs(or normal saline)via intragastric administration once daily for a duration of 30 days.Except the control group,chronic pharyngitis was induced in rats of the other groups by usingβ-hemolytic streptococcus.Following euthanasia,serum inflammatory levels of interleukin-6(IL-6),cyclooxygenase-2(COX-2),interleukin-1β(IL-1β),intercellular adhesion molecule-1(ICAM-1),C-reactive protein(CRP),tumor necrosis factor(TNF-α),monocyte chemoattractant protein-1(MCP-1),and prostaglandin E2(PGE2)were measured.Additionally,pharyngeal tissues were stained with HE and pathological characteristics were observed.Results Toxicological studies have demonstrated that the administration of Panacis Major's Rhizoma resulted in significant increase in plasma alanine transaminase levels and spleen index of mice,along with corresponding tissue pathological alterations.Nevertheless,no noteworthy pathological changes were observed in other organs,and there were no notable changes in blood routine and plasma biochemical indicators.Pharmacodynamic investigations have revealed that Panacis Maioris Rhizoma effectively reduces the serum levels of inflammatory factors and improves pathological changes in pharyngeal tissues.Conclusion Panacis Maioris Rhizoma alleviatedβ-hemolytic streptococcus-induced CP by inhibiting inflammatory responses,and may show potential toxicity to the spleen.