基于代谢组学解析‘翠碧一号’烟叶烘烤过程中多酚类代谢物动态变化

Polyphenol Metabolites During Flue-curing in Tobacco‘Cuibi NO.1’Leaves:Dynamic Changes Based on Metabolomics Analysis

为了解析烟叶烘烤过程中影响烟叶调制特性和香气品质的多酚类代谢物的动态变化,以‘翠碧一号’上部叶为材料,采集烘烤过程中5个关键节点15份样品,采用超高液相色谱串联质谱检测分析代谢物,通过K均值进行差异代谢物聚类,利用KEGG数据库进行注释和代谢通路分析。从15份样品中共鉴定出1069种代谢物,主要为黄酮类、酚酸类,脂质、生物碱、氨基酸等物质,其中多酚类为374种,达总数的34.99%。差异代谢物及其K均值聚类分析显示多酚类代谢物含量变化主要发生在变黄期至定色初期,代谢物富集在酪氨酸代谢,苯丙氨酸代谢、苯丙烷类和类黄酮生物合成通路中,差异代谢物在烟叶风味和品质相关的氢醌、绿原酸、山奈酚和芥子酸等物质的生物合成途径中显著富集。基于代谢组学解析了烟叶烘烤过程中的多酚类代谢物变化规律,为揭示烟草品质形成的物质基础和调控棕色化反应奠定基础。

In order to reveal dynamic changes of polyphenol metabolites that influence the modulation characteristics and aroma quality of tobacco leaves during curing process,using the upper leaves of tobacco variety of‘Cuibi NO.1’(CB-1)as the material,a total of 15 samples during curing process were collected and analyzed by UPLC-MS/MS.Cluster analysis of the differential metabolites was conducted based on K-means,and the metabolites annotation and metabolic pathway analysis were conducted based on KEGG database.A total of 1069 metabolites were identified from 15 samples,including flavonoids,phenolic acids,lipids,alkaloids,amino acids etc.Notably,374 of polyphenols were identified,accounting for 34.99%of the total metabolites.The differential metabolites and their K-means cluster analysis showed that most significant changes in metabolite content occurred during the transition from the yellowing stage to the early color-fixing stage.Furthermore,these metabolites were found to be enriched in pathways such as tyrosine metabolism,phenylalanine metabolism,phenylpropanoid biosynthesis,and flavonoid biosynthesis.Additionally,the differential metabolites were found to be enriched in the biosynthesis pathways of hydroquinone,chlorogenic acid,kaempferol,and sinapic acid,which were closely related to tobacco flavor and quality.The dynamic changes of polyphenol metabolites during the tobacco leaf flue-curing process were illuminated based on the metabolomics,and these findings lay a foundation for understanding the material basis of tobacco quality formation and the regulation of tobacco browning.