基于网络药理学的化妆品原料粉防己抗敏作用机制分析

Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology

采用网络药理学方法探索化妆品原料粉防己对敏感肌肤的抗敏作用机制。通过TCMSP、中国知网、PubMed等数据库收集粉防己的主要化学成分,并利用SwissTargetPrediction预测其靶点,与从Genecards数据库获得的疾病靶点取交集后采用String数据库构建蛋白质相互作用(PPI)网络,采用Cytoscape3.9.1软件分析获得核心靶点,再运用DAVID在线数据库对核心靶点进行基因GO功能分析和KEGG通路富集分析,Cytoscape3.9.1软件构建粉防己活性成分-靶点交互网络。共筛选出粉防己潜在活性成分50个及相应靶点682个,敏感肌肤疾病相关靶点2389个,交集靶点295个,其中核心靶点39个。GO富集分析结果显示,39个核心靶点主要涉及蛋白磷酸化、信号转导、转录调控、基因表达等多种生物学过程。KEGG通路富集分析结果显示,粉防己发挥抗敏作用可能与VEGF信号通路、HIF-1信号通路、ErbB信号通路、PI3K-Akt信号通路密切相关。粉防己中荷包牡丹碱、千金藤素、防己诺林碱等活性成分可能是通过作用于JAK2、JAK3、SYC等靶点发挥抗敏作用,体现了粉防己多成分-多靶点-多通路的作用特点。

Explore the mechanism of anti�sensitive skin effect of cosmetic raw material Stephania tetrandra on sensitive skin using a network pharmacological approach.The main chemical components of Stephania tetrandra were collected through TCMSP,CNKI,PubMed and other databases,and their targets were predicted using Swiss Target Prediction,intersected with the disease targets obtained from Genecards database and then used String database to construct protein interaction(PPI)network.Cytoscape3.9.1 software was used to analyze the core targets,and the DAVID online database was used to perform GO functional analysis and KEGG pathway enrichment analysis on the core targets.The results of GO enrichment analysis show that the 39 core targets are mainly involved in various biological processes such as protein phosphorylation,signal transduction,transcriptional regulation and gene expression.The results of KEGG pathway enrichment analysis show that the anti-sensitive skin effect of Stephania tetrandra may be closely related to VEGF signaling pathway,HIF-1 signaling pathway,ErbB signaling pathway and PI3K-Akt signaling pathway.The active ingredients in Stephania tetrandra,such as Dicentrined,Cepharanthine and Fangchinoline,may exert their anti-sensitive skin effects by acting on the targets of JAK2,JAK3 and SYC,reflecting the multicomponent�multitarget-multipathway action characteristics of Stephania tetrandra.