莲藕解酒功能软糖的制备及功效评价

Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge

为了探讨了莲藕解酒成分的提取工艺条件及其制备的软糖的解酒功效。本研究以莲藕为原料,以乙醇脱氢酶(ADH)体外激活率为指标,通过单因素和正交试验对提取溶剂、料液比、提取时间和提取温度进行研究。对最优提取工艺下的莲藕提取物进行成分分析并以此作为原料制备解酒功能软糖,测定软糖中添加不同浓度莲藕提取液对DPPH自由基、ABTS+自由基和·OH自由基的清除率;建立小鼠醉酒模型评价莲藕功能软糖的醒酒功效。实验结果表明,莲藕解酒成分最佳提取工艺为溶剂选用100%甲醇,料液比1∶40,提取温度60℃,提取时间5 h,此工艺下得到的莲藕提取物对ADH体外激活率为(19.60±2.10)%。UPLC-QTOF-MS/MS分析此工艺条件下莲藕提取物有433种成分,主要物质为酚酸和黄酮类物质。莲藕解酒功能软糖对ABTS+自由基、·OH自由基和DPPH自由基清除率的IC50分别为14.59、101.96和5.54 mg·mL^(-1)。动物实验证明莲藕解酒功能软糖可以显著提高(P<0.05)饮酒后小鼠肝脏ADH活性并显著降低(P<0.05)小鼠饮酒后血液中乙醇的含量,同时延长小鼠平均醉酒潜伏时间并缩短醉酒后翻正反射恢复时间。表明莲藕解酒软糖具有一定的解酒效果。本研究结果为开发具有解酒护肝功效的功能性食品提供了新的理论和实践基础。

This paper focuses on the extraction process conditions of the antidote components of lotus roots and the antidote efficacy of the fudge prepared.The extraction solvents,solid-liquid ratio,extraction time and extraction temperature were investigated by single-factor and orthogonal experiments using lotus root as the raw material and the in vitro activation rate of alcohol dehydrogenase(ADH)as the index.The composition of the lotus root extract was analyzed and used as the raw material for the preparation of alcoholic functional gummies.The scavenging rates of DPPH radicals,ABTS+radicals and·OH radicals were determined by adding different concentrations of lotus root extracts to the gel fudge.The results showed that the best extraction process of lotus root extract was 100%methanol,1∶40 ratio,60℃and 5 h.The in vitro activation rate of ADH was(19.60±2.10)%,and 433 components were analyzed by UPLC-QTOF-MS/MS,the main substances are phenolic acids and flavonoids.The IC 50 of lotus root gummy extract for the scavenging of ABTS+radical,·OH radical and DPPH radical were 14.59 mg·mL^(-1),101.96 mg·mL^(-1)and 5.54 mg·mL^(-1),respectively.Animal experiments demonstrated that lotus root relieving alcoholism functional gel fudge significantly increased(P<0.05)and decreased(P<0.05)the liver ADH activity in mice after alcohol consumption.It also prolonged the mean latency time of intoxication and shortened the recovery time of the reflex after intoxication.The results of this study suggest that lotus root relieving alcoholism functional gel fudge have certain antidrinking effects.The results of this study provide a new theoretical and practical basis for the development of functional food with the effect of alcohol detoxification and liver protection.