固定化抑藻微生物的制备及抑藻活性研究

Preparation Characterization of the Immobilized Algae Inhibiting Microorganism

为探究固定化技术对一株抑藻微生物暹罗芽孢杆菌抑藻活性的影响,选择6种包埋材料组合制备了固定化微球,对微球物理性质、抑藻性能进行了分析.结果发现,利用海藻酸钠(2%)-聚乙烯醇(6%)-活性炭(1%)制备的固定化暹罗芽孢杆菌微球具有较好的机械强度和传质性能,培养4 d后对球形棕囊藻的抑制率达到(78.87±4.88)%.实验还发现,固定化暹罗芽孢杆菌微球表现出与游离菌株相似的长期抑藻能力,预先活化的固定化微球经过5次重复使用,对藻生长抑制率为(76.34±2.21)%.

In order to investigate the effect of immobilization on the inhibitory activity of an algicidal microorganism,Bacillus Siamensis 0910-33,this study prepared immobilized microspheres in six combinations,and then analyzed the physical properties and inhibitory performance of the microspheres.The results showed that the immobilized microspheres(SA-PVA-AC)prepared with 2%sodium alginate 6%polyvinyl alcohol 1%activated carbon had better mechanical strength and mass transfer performance than the others,and the inhibition rate reached(78.87±4.88)%after 4 days of co culture with Phaeocystis globosa.It was also found that SA-PVA-AC exhibited a similar long-term inhibitory ability to the free strain,and more importantly,the pre-activated immobilized microspheres could still maintain an inhibition rate of(76.34±2.21)%against microalgae growth after five repeated uses.The results provide technical support for the application of immobilized algae-lysing microorganisms in algal bloom control.