基于MRI研究血清GH、8-OHdG、HMGB1与新生儿缺氧缺血性脑病临床分度的相关性

MRI-based study of the correlation between serum GH,8-OHdG,HMGB1 and clinical classification of neonatal hypoxic-ischemic encephalopathy

目的 研究血清生长素(GH)、8-羟基脱氧鸟苷(8-OHdG)、高迁移率族蛋白(HMG)B1与新生儿缺氧缺血性脑病(HIE)临床分度的相关性。方法 选取2019年1月至2022年12月在秦皇岛市第一医院进行诊断并治疗的120例HIE患儿作为研究组,另选取同期健康新生儿120例作为对照组,比较研究组及对照组、不同临床分度及不同磁共振成像(MRI)评分的血清GH、8-OHdG、HMGB1的差异。采用Spearman相关性分析研究对象临床分度、MRI评分与血清GH、8-OHdG、HMGB1水平的相关性。结果 研究组血清GH低于对照组,8-OHdG、HMGB1高于对照组,差异有统计学意义(t值分别为22.828、48.563、98.440,P<0.05);不同病情患儿的血清GH、8-OHdG、HMGB1水平及MRI评分差异存在统计学意义,进一步两两比较,患儿的血清GH、HMGB1水平从高到低依次为轻度组、中度组及重度组,8-OHdG及MRI评分从高到低依次为重度组、中度组及轻度组(F值分别为13.055、13.150、12.710、14.205,P<0.05);不同MRI评分患儿血清GH、8-OHdG、HMGB1水平差异存在统计学意义,进一步两两比较,患儿的血清GH、HMGB1水平从高到低依次为0~3分组、4~7分及8~10分组,8-OHdG从高到低依次为8~10分组、4~7分组及0~3分组(F值分别为15.432、13.674、19.076,P<0.05);相关性分析结果显示,患儿的临床分度、MRI评分与GH、HMGB1呈负相关性(r值分别为-0.738、-0.666,-0.579、-0.671,P<0.05),与8-OHdG呈正相关性(r值分别为0.617、0.690,P<0.05)。结论 血清GH、8-OHdG、HMGB1与新生儿缺氧缺血性脑病临床分度呈现显著相关性,可作为临床诊断的重要依据。

Objective To investigate the correlation between serum growth hormone(GH),8-hydroxydeoxyguanosine(8-OHdG),and high mobility group protein(HMG)B1 and clinical grades of neonatal hypoxic-ischemic encephalopathy(HIE).Methods 120 children with HIE who were diagnosed and treated in the First Hospital of Qinhuangdao from January 2019 to December 2022 were selected as the study group,and another 120 healthy newborns in the same period were selected as the control group,and the differences in serum GH,8-OHdG,and HMGB1 were compared between the study group and the control group,and between different clinical grades and different magnetic resonance imaging(MRI)scores.Spearman s correlation was used to analyse the correlation between clinical grades,MRI scores and serum GH,8-OHdG,HMGB1 levels in the study subjects.Result The serum GH of the study group was lower than that of the control group,and 8-OHdG and HMGB1 were higher than that of the control group,with statistically significant differences(t=22.828,48.563,and 98.440,respectively,P<0.05).There were statistically significant differences in serum GH,8-OHdG,HMGB1 levels and MRI scores among children with different conditions,and further two-by-two comparisons were made,with the children s serum GH and HMGB1 levels ranging from high to low for the mild group,the moderate group,and the severe group,and the 8-OHdG and MRI scores ranging from high to low for the severe,moderate,and the mild group(F=13.055,13.150,12.710,14.205,respectively,P<0.05).There were statistically significant differences in serum GH,8-OHdG,and HMGB1 levels among children with different MRI scores,and further two-by-two comparisons were made,and the children s serum GH and HMGB1 levels were grouped in 0-3,4-7,and 8-10 in descending order,and 8-OHdG was grouped in 8-10,4-7,and 0-3 in descending order(F=15.432,13.674,19.076,respectively,P<0.05).The results of correlation analysis showed that the children s clinical scores and MRI scores were negatively correlated with GH and HMGB1(r=-0.738,-0.666,-0.579,-0.671,respectively,P<0.05)and positively correlated with 8-OHdG was positively correlated(r=0.617,0.690,respectively,P<0.05).Conclusion Serum GH,8-OHdG,and HMGB1 show significant correlation with the clinical classification of neonatal hypoxic-ischemic encephalopathy,which can be used as an important basis for clinical diagnosis.