莓叶委陵菜叶插繁殖技术研究

A study on leaf cutting propagation technology for Potentilla fragarioidis

莓叶委陵菜(Potentilla fragarioidis)作为我国北方地区极具开发潜力的乡土地被植物,存在着有性繁殖材料不易获得且繁殖系数较低的问题。而扦插繁殖作为一种无性繁殖手段,能为莓叶委陵菜的快速繁殖提供新的途径。本研究以莓叶委陵菜生长健壮、无病虫害的叶为试验材料,通过正交试验针对不同扦插基质、激素种类及其浓度对莓叶委陵菜叶插生根效果的影响进行探究。结果表明,莓叶委陵菜插穗在扦插45 d后能够形成根系,不同处理水平的扦插基质、激素种类及其浓度间的交互效应对插穗生根效果的影响具有显著差异(P<0.05)。扦插基质是影响莓叶委陵菜叶插生根率的主导因子,而激素种类是影响平均单株生根数、平均单株总根长、最长根长和根系效果指数的主要因素。以草炭+珍珠岩+蛭石(1:1:1)为基质,扦插前使用300 mg·L^(-1)的IBA对插穗进行处理生根效果最佳,该处理方式下扦插生根率达63.33%,平均单株生根数为6.90条,平均单株总根长为27.98 cm,最长根长13.83 cm,根系效果指数达20.50。本研究结果可为莓叶委陵菜无性系繁殖体系的快速建立提供理论依据。

Potentilla fragarioidis is a rural cover plant with excellent developmental potential in northern China.However,materials for its sexual propagation are not readily available,and the reproductive coefficient is low.As a means of asexual reproduction,propagation via cuttings may provide a new means of rapidly producing P.fragarioidis.In this study,robust and disease-free leaves of P.fragarioidis were used,and the effects of different cutting substrates,hormone types,and their concentration on the rooting of P.fragarioidis leaf cuttings were investigated in an orthogonal experiment.The results showed that the root system of cuttings form after 45 days.Significant differences in the rooting effect of cuttings were observed due to the interaction effects between the cutting substrate,hormone type,and their concentrations at different treatment levels(P<0.05).Cutting substrate was the dominant factor affecting rooting rate,and hormone type was the main factor affecting the average number of roots per plant,mean total length of roots per plant,most extended root length,and root effect index.The best rooting effect was obtained using 300 mg·L^(-1) IBA as the cutting treatment hormone and peat+perlite+vermiculite(1꞉1꞉1)as the cutting substrate.The rooting rate of cuttings under this treatment reached 63.33%;the average number of roots per plant was 6.90;the average root length was 27.98 cm;the most extended root length was 13.83 cm;and the root effect index was 20.50.These experimental results provide a theoretical basis for the rapid establishment of a propagation system for P.fragarioidis.