补肾中药调控骨髓间充质干细胞成骨分化的RNA m6A修饰作用研究

Effects of Kidney-tonifying Herbs on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells from the Perspective of m6A Modification

骨髓间充质干细胞(BMSCs)是一种成体干细胞,具有成骨细胞分化能力。BMSCs成骨分化能力减弱易导致骨质疏松等多种骨相关疾病。中医学认为“肾主骨生髓”,补肾中药是治疗骨质疏松的常用药物,具有调控BMSCs成骨分化作用。腺苷N6位甲基化修饰(m6A)是真核生物RNA的转录后修饰,是BMSCs成骨分化的关键因素。探讨m6A动态修饰与BMSCs成骨分化的关系,分析补肾中药调控m6A修饰的可能作用及靶点,能够为补肾中药治疗骨质疏松提供新靶点,为中医肾藏象理论的科学阐释提供思路。

Bone marrow mesenchymal stem cells(BMSCs) are adult stem cells with osteogenic differentiation ability.The weakening osteogenic differentiation ability of BMSCs can easily lead to a variety of bone-related diseases,such as osteoporosis.Based on the theory of traditional Chinese medicine that “kidney governs the bone and generates the marrow”,kidney-tonifying herbs are commonly used in the treatment of osteoporosis,which has the effect of regulating osteogenic differentiation of BMSCs.N6-methyladenosine(m6A) is a post-transcriptional modification of eukaryotic RNA and a key factor in osteogenic differentiation of BMSCs.This study explores the relationship between m6A dynamic modification and osteogenic differentiation of BMSCs,and analyzes the possible role and targets of kidney-tonifying herbs in regulating m6A modification,which can provide new targets for kidney-tonifying herbs in the treatment of osteoporosis,and provide ideas for the scientific interpretation of the theory of visceral manifestation of kidney in traditional Chinese medicine.