崂山奶山羊不同泌乳期乳腺组织可变剪接的鉴定与分析

Identification and Analysis of Mammary Gland Tissues Alternative Splicing at Different Lactation Stages of Laoshan Dairy Goats

本研究对崂山奶山羊泌乳初期、高峰期和末期乳腺组织RNA-Seq数据进行可变剪接分析,共鉴定到151503个可变剪接事件,对应17627个可变剪接基因,6323个差异剪接基因,显著富集到细胞器、蛋白质结合、mRNA加工和RNA剪接等相关GO条目,主要参与催产素信号通路、钙信号通路、内质网中的蛋白质加工等,与乳腺组织生理活性密切相关的KEGG通路,筛选出PTEN作为参与乳腺发育及泌乳相关生理过程中调控网络的核心基因。研究结果表明,可变剪接在奶山羊不同泌乳期的乳腺组织具有发育阶段特异性,特有可变剪接基因与乳腺组织生理活性特点密切相关,在调控奶山羊乳腺组织发育及泌乳生理中发挥重要作用。本研究可为探究可变剪接机制调控奶山羊乳腺发育与泌乳性状的分子机理提供理论依据。

Based on the identification and analysis of alternative splicing genes from RNA-Seq of the mammary gland tissue of Laoshan dairy goats at the early lactation,peak lactation and late lactation,a total of 151503 alternative splicing events,corresponding to 17627 alternative splicing genes,and 6323 differentially splicing genes,significantly enriched to related GO entries including organelles,protein binding,mRNA processing and RNA splicing,and mainly involved in oxytocin signaling pathway,calcium signaling pathway,and protein processing in the endoplasmic reticulum,and KEGG pathway closely related to the physiological activity of breast tissue,and screened PTEN as the core gene involving in the regulation network of mammary gland development and lactation-related physiological processes.The results indicated that variable splicing has developmental stage specificity in mammary gland tissues of dairy goats at different lactation stages,the unique variable splicing gene is closely related to the physiological activity of mammary gland tissue and plays an important role in regulating the development of mammary gland tissue and lactation physiology of dairy goats.Our study can provide the theoretical basis for exploring the molecular mechanism of alternative splicing mechanisms regulating mammary gland development and lactation traits in dairy goats.