蜡样芽孢杆菌GW-01全基因组测序及生物学特性分析

Whole Genome Sequencing and Biological Characterization of Bacillus cereus GW-01

目的:通过全基因组测序和生物信息学,研究前期筛选可降解高效氯氰菊酯(β-CY)蜡样芽孢杆菌GW-01的基因组序列信息和生物学特性,为其安全性评估提供参考。方法:利用HPLC验证了GW-01降解β-CY的能力。GW-01的整个基因组基于二代Illumina NovaSeq与三代PacBio Sequel测序平台相结合的测序技术,对菌株GW-01进行全基因组测序,并对测序数据进行基因组组装、基因预测与功能注释、碳水化合物活性酶预测、毒力因子和抗生素抗性分析,此外,还基于gyrA基因序列对菌株GW-01构建系统发育树。结果:GW-01在第6 d可降解48.4%的β-CY;GW-01菌株全基因组大小为5244145 bp,基因组类型为环状,平均GC含量36.43%,共编码5314个基因,含有104个tRNA基因,313个ncRNA,GW-01菌株全基因组序列在GO、KEGG、COG、NR和Swiss-Prot、CAZy、VFDB、CARD数据库分别注释到基因3536、4714、4434、5290、3854、135、263和96个,GW-01与其他四个近缘菌株相比显示出高GC含量,并且毒力因子和耐药基因较少。结论:GW-01与其近缘菌相比,显示出明显不同的进化途径和毒素编码基因,表明其安全性较高。

Objective:To analyze the genome sequence information and biological properties of the pre-screened high-performance cypermethrin(β-CY)-degrading Bacillus cereus strain GW-01 through whole genome sequencing and bioinformatics,and to provide valuable insights for its safety assessment.Methods:The degradation ability of GW-01 towardsβ-CY was verified using HPLC.The whole genome of strain GW-01 was sequenced using third-generation single-molecule sequencing technology based on the second-generation Illumina NovaSeq and third-generation PacBio Sequel sequencing platforms.The sequencing data underwent analyses for genome assembly,gene prediction,functional annotation,prediction of carbohydrate-activating enzymes,virulence factors,and antibiotic resistance.Additionally,a phylogenetic tree was constructed for strain GW-01 based on the gyrA gene sequence.Results:On day 6,GW-01 degraded 48.4%ofβ-CY.The whole genome size of GW-01 strain was 5244145 bp with a cyclic genome type and an average GC content of 36.43%.It encoded a total of 5314 genes,including 104 tRNA genes and 313 ncRNAs.The whole genome sequences of GW-01 were annotated in the GO,KEGG,COG,NR,Swiss-Prot,CAZy,VFDB,and CARD databases,yielding 3536,4714,4434,5290,3854,135,263 and 96 annotated genes,respectively.GW-01 exhibited higher GC content and fewer virulence factors and resistance genes compared to the other four closely related strains.Conclusion:GW-01 displayed a distinct evolutionary pathway and a lower number of toxin-encoding genes compared to common Bacillus cereus strains,suggesting its higher safety profile.