老年慢性心力衰竭患者血清微小RNA-665和微小RNA-144表达水平与心功能的关系

Relationship between serum miR-665 and miR-144 levels and cardiac function in elderly patients with chronic heart failure

目的探讨老年慢性心力衰竭(chronic heart failure,CHF)患者血清微小RNA(miR)-665、miR-144水平与心功能的关系。方法连续选取2021年3月至2023年3月内蒙古医科大学附属医院诊治的老年CHF患者120例为CHF组,根据纽约心脏病协会(NYHA)心功能分级:Ⅱ级39例、Ⅲ级51例、Ⅳ级30例;选择同时间段老年健康志愿者120例纳入对照组。收集临床资料及心功能指标,检测血清miR-665、miR-144表达水平,Pearson相关性分析miR-665、miR-144与心功能指标的关系。结果与对照组比较,CHF组LVEF显著降低,左心室舒张末期内径(left ventricular end-diastolic diameter,LVEDD)、左心室收缩末期内径(left ventricular end-systolic diameter,LVESD)及血清miR-665、miR-144表达水平显著升高(P<0.01)。CHF组心功能Ⅱ、Ⅲ、Ⅳ级患者LVEF依次降低,LVEDD、LVESD及血清miR-665、miR-144表达水平依次升高,差异有统计学意义(P<0.01)。Pearson相关性分析显示,血清miR-665与miR-144呈正相关(r=0.693,P=0.000);miR-665、miR-144与LVEF呈负相关(r=-0.577,P=0.000,r=-0.591,P=0.000),与LVEDD(r=0.485,P=0.000,r=0.507,P=0.000)、LVESD(r=0.539,P=0.000,r=0.494,P=0.000)呈正相关。结论老年CHF患者血清miR-665、miR-144水平升高,均与心功能密切相关。

Objective To investigate the serum levels of miR-665 and miR-144 in the elderly patients with chronic heart failure(CHF)and their relationship with cardiac function.Methods A total of 120 elderly CHF patients who were diagnosed and treated in our hospital from March 2021 to March 2023 were collected and then divided into NYHA gradeⅡ(n=39),Ⅲ(n=51)andⅣ(n=30)subgroups according to the results of NYHA classification.Another 120 elderly healthy volunteers during the same period were recruited as the control group.Clinical data and cardiac function indicators were collected,and the expression levels of miR-665 and miR-144 in serum were detected.Pearson correlation analysis was applied to analyze the relationship of miR-665 and miR-144 levels with cardiac function indicators.Results The CHF group had significantly decreased LVEF,increased left ventricular end-diastolic diameter(LVEDD)and left ventricular end-systolic diameter(LVESD),and elevated serum levels of miR-665 and miR-144 than the control group(P<0.01).Sequentially reduced LVEF and raised LVEDD and LVESD values and serum miR-665 and miR-144 levels were observed in the patients with NYHA gradesⅡ,Ⅲ,andⅣin turn(P<0.01).Pearson correlation analysis showed that there was a positive correlation of the serum level of miR-665 with that of miR-144 in CHF patients(r=0.693,P=0.000),of the miR-665 and miR-144 levels with LVEDD(r=0.485,r=0.507,P<0.01)and LVESD(r=0.539,r=0.494,P<0.01),and a negative correlation of the serum levels with LVEF(r=-0.577,r=-0.591,P<0.01).Conclusion The serum levels of miR-665 and miR-144 are elevated in elderly CHF patients,and are closely associated with their cardiac function.