灵芝三萜提取工艺的优化

Extraction Process Optimization of Ganoderma Triterpenes

以灵芝孢子粉为原料,使用体积分数为70%的乙醇为提取剂,采用酶解与超声辅助提取相结合的方法,将不同液固比、超声时间、酶解时间和酶用量设定为4个因素,进行单因素试验并设计响应面试验,以确定最优提取方式及其影响因素.利用大孔树脂层析法对灵芝三萜进行分离纯化,通过优化分离纯化工艺,确定最佳洗脱树脂、洗脱液体积分数、上样液流速以及上样液质量比.采用高效液相色谱法分析灵芝总三萜的组分差异.通过预实验分析,与单一提取法相比,酶+超声辅助提取更高效.采用乙醇为提取剂提取灵芝中三萜类化合物可提高三萜的纯度.最优条件下可实现对三萜含量的快速、精确测定,为灵芝三萜的分离纯化提供理论依据.

Ganoderma lucidum spore powder was used as raw material,ethanol with a volume fraction of 70%as extractant.We adopted a combination of enzymatic hydrolysis and ultrasound assisted extraction method,set different liquid-solid ratios,ultrasound time,enzymatic hydrolysis time,and enzyme dosage as four factors for a one-way test and designed a response surface experiment to determine the optimal extraction method and its influencing factors.The Ganoderma triterpene were separated and purified by using macroporous resin chromatography.By optimizing the separation and purification process,the optimal elution resin,eluent volume fraction,flow rate of the upper sample solution and the mass ratio of the upper sample solution were determined.The compositional differences of the total Ganoderma triterpenes were analysed by high performance liquid chromatography(HPLC).Though the pre-experimental analysis,the results show that the enzyme+ultrasound assisted extraction is more efficient compared to the single extraction method.Ethanol is used as an extractant to extract triterpenoids from Ganoderma lucidum can enhance the purity of triterpenoids.Under optimal conditions,the rapid and accurate determination of the triterpene content can be achieved,providing a theoretical basis for the separation and purification of Ganoderma triterpenes.