KRAS基因3'UTR多态性与云南汉族人群宫颈癌及宫颈上皮内瘤变的相关性

Correlation of KRAS Gene 3′UTR Polymorphisms with Cervical Cancer and Cervical Intraepithelial Neoplasia in Chinese Han Population in Yunnan Province

目的探究KRAS基因中3′U TR区域的rs712和rs7973450位点与云南汉族人群宫颈癌(cervical cancer,CC)和宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)的相关性。方法共纳入CIN患者461例、CC患者961例及其健康对照983例,采用TaqMan探针法进行基因分型检测,并分析2个SNP位点与CIN及CC的相关性。结果rs7973450位点的A等位基因可能是CIN(P=0.004,OR=0.651,95%CI 0.487~0.871)与CC(P=7.00×10-4,OR=0.667,95%CI 0.529~0.844)发生的保护性因素。rs712位点在CIN组、CC组和对照组间等位基因和基因型分布频率的差异无统计学意义(P>0.017);单倍型分析的结果显示,单倍型rs712Ars7973450G与更高的CIN(P=4.00×10^(-4),OR=1.714,95%CI1.269~2.314)和CC(P=3.84×10^(-5),OR=1.667,95%CI 1.305~2.131)发生风险相关;单倍型rs712A-rs7973450A则与更低的CC发生风险相关(P=0.012,OR=0.790,95%CI 0.658~0.950)。结论位于KRAS基因3U′TR区域的SNP位点rs7973450的A等位基因可能是云南汉族人群CIN和CC发生的保护性因素。

Objective To investigate the correlation between rs712 and rs7973450 located at the 3′UTR region of the KRASgene and the risk of cervical cancer(CC)and cervical intraepithelial neoplasia(CIN)in Chinese Han population in Yunnan province.Methods A total of 2405 individuals(461 subjects with CIN,961 subjects with CC and 983 healthy controls)were enrolled.The SNPs were genotyped used TaqMan assay and the correlation of these SNPs with CIN and CC was analyzed.Results The A allele of rs7973450 might be a protective factor for the occurrence of CIN(P=0.004,OR=0.651,95%CI 0.487~0.871)and CC(P=7.00×10^(-4),OR=0.667,95%CI0.529~0.844).There was no significant difference in allelic and genotypic distribution of rs712 among CIN,CC and Control groups(P>0.017).The haplotype assay showed thatrs712A-rs7973450G was associated with increased risk of CIN(P=4.00×10^(-4);OR=1.714,95%CI1.269~2.314)and CC(P=3.84×10-5,OR=1.667,95%CI1.305~2.131).While haplotype rs712A-rs7973450A was associated with a lower risk of CC(P=0.012,OR=0.790,95%CI0.658~0.950).Conclusion The A allele of rs7973450 in 3′UTR of KRASgene might be the protective factor for the occurrence of CIN and CC in a Chinese Han population in Yunnan province.