郑州市城市污水中新型冠状病毒监测结果

Monitoring results of SARS-CoV-2 in urban sewage in Zhengzhou city

目的分析河南省郑州市环境污水中新型冠状病毒监测结果,为环境监测工作提供数据支持,初步探索环境监测在新型冠状病毒感染疫情防控中的作用。方法选择郑州市2家污水处理厂作为监测点,2023年3—7月,每周每个污水处理厂各采集2份污水,用PEG法进行浓缩富集,应用多重实时荧光逆转录-聚合酶链式反应(RT-PCR)方法对新型冠状病毒进行检测和分析,选取新型冠状病毒测序获得全基因组序列,应用分析软件判断病毒型别。结果城市污水中新型冠状病毒总检出率为89.09%。3—4月份,新型冠状病毒检出率分别76.47%、81.82%,5—7月份检出率均为100.00%。在采集的55份污水中,污水原液、沉渣、上清液、浓缩样本中均有新型冠状病毒检出,病毒含量大小为浓缩样本>原液>上清液>沉渣,N基因计算出浓缩样本中新冠病毒浓度是污水原液中的13.57倍,城市污水中新冠病毒浓度在第23周达到高峰,随后开始下降,第26周降到低点。5月份病毒分型污水中占比前两位的分别为XBB.1.9、XBB.1.16及其亚分支。结论郑州市环境污水中新型冠状病毒的检出率较高,尤其是5—7月份,病毒分型为奥密克戎XBB亚分支,环境监测可以作为医院病例监测的补充。

Objective To analyze the monitoring results of SARS-CoV-2 in environmental sewage in Zhengzhou city,Henan province,provide data support for environmental monitoring,and preliminarily explore the role of environmental monitoring in COVID-19 prevention and control.Methods Two sewage treatment plants in Zhengzhou city were selected as monitoring sites.From March-July 2023 each sewage treatment plant collected two samples of sewage per week;the enrichment was conducted by PEG method.Multiple real-time fluorescence reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect and analyze novel coronavirus;the whole genome sequence was obtained by SARS-CoV-2 sequencing,and the virus type was judged by analysis software.Results The total detection rate of novel coronavirus in urban sewage was 89.09%.From March to April,the detection rate of novel coronavirus was 76.47%and 81.82%respectively,and from May to July,the detection rate was 100.00%.Of the 55 sewage samples collected,SARS-CoV-2 was detected in sewage solution,sediment,supernatant and concentrated samples.Virus content size:concentrated sample>stock solution>supernatant>sediment.N gene calculation showed that the concentration of SARS-CoV-2 in concentrated samples was 13.57 times that in sewage solution.The concentration of SARS-CoV-2 in urban sewage peaked in the 23rd week,then began to decline,and then dropped to a low point in the 26th week.In May,the top two viruses in sewage were XBB.1.9,XBB.1.16 and its sub-branches.Conclusions The detection rate of SARS-CoV-2 in environmental sewage in Zhengzhou is high,especially from May to July.The virus type is Omicron XBB subbranch,and environmental monitoring can be used as a supplement to hospital case monitoring.