泻白散质量标志物的发现及其作用机制预测分析

Discovery of Q-markers of Xiebai Powder and Predictive Analysis of Its Mechanism of Action

目的:旨在明确泻白散质量标志物并预测其作用机制,确保临床疗效。方法:通过Q-markers“五原则”筛选泻白散质量标志物;采用TCMSP、Swiss Target Prediction等数据库获取Q-markers潜在作用靶点,构建质量标志物的蛋白互作网络。通过KEGG、GO、拓扑富集分析构建“关键靶点-关键通路-关键生物过程”网络,进而预测泻白散质量标志物的潜在作用机制。结果:研究显示,氧化白藜芦醇、绿原酸、阿魏酸、甘草素、甘草酸和芒柄花素6个化合物可作为泻白散的潜在质量标志物,这些物质可能通过调节炎症反应中的一氧化氮生物合成过程、AGEs-RAGE信号通路等途径发挥作用。结论:通过初步探讨泻白散的质量标志物及其作用机制,为泻白散质量控制与临床应用提供科学依据。

Objective:To identify Q-markers and predict their mechanisms of action to ensure clinical effi cacy.Methods:The quality markers for Xiebai Powder were screened by Q-markers"Five Principles".Databases such as TCMSP and Swiss Target Prediction were used to obtain potential targets of Q-markers,in order to construct a protein interaction network for Q-markers.Through KEGG,GO,and topological enrichment analysis,the"key target-key signal pathway-key biological process"network was constructed to predict the potential mechanism of the Q-markers of Xiebai Powder.Results:This study showed that oxyresveratrol,chlorogenic acid,ferulic acid,liquiritigenin,glycyrrhizic acid and formononetin could be used as potential quality markers of Xiebai Powder.These substances might play a role in regulating the nitric oxide biosynthesis process and AGEs-RAGE signaling pathway in inflammatory response.Conclusion:This article preliminarily explores the quality markers and mechanism of Xiebai Powder,aiming to provide scientifi c basis for the quality control and clinical application of Xiebai Powder.