摘要
[目的]评估交配形式对蕨类繁殖的影响。[方法]以姬蕨(Hypolepis punctata)为例,设计配子体单独培养(同配自交)和混合培养(混交)比较试验,比较两种交配系统下孢子体萌发状态(萌发率、存活率),孢子体成长情况及生长速率。[结果]尽管自交幼苗生长趋势弱于混交个体,单独培养产生的完全纯合的姬蕨孢子体最终存活率达到62.5%,说明蕨类的自交确实可以导致近交衰退,但仍然具有可接受的存活能力,单个孢子仍有很大概率上延续种群。蕨类植物由于其特殊的生活史特性,可通过单独培养得到完全纯合的自交个体,从而得到性状稳定的品系。[结论]该研究中单独培养技术在园艺育种方向具有很好的应用前景。
[Objective]The aim was to evaluate the influence of mating pattern on fern reproduction.[Method]Taking Hypolepis punctata as a case,a comparative experiment of separately and mixed culturing of gametophytes was designed to compare the germination rate,survival rate,sporophyte growth and growth rate of sporophyte under two mating systems.[Result]The final survival rate of the completely homozygous sporophyte produced by separately culturing reached 62.5%,although their growth performance was weaker than that of mixed-crossing individuals.It suggests that selfing ferns can indeed lead to inbreeding depression,but still have a high ability to survive,which make a single spore has a high probability of continuing the population.Due to the special life-history characteristics of fern,we can obtain completely homozygous selfing individuals as trait-stable strains through separately culturing.[Conclusion]This study proved that the separately culturing technique is applicable in horticultural breeding.
作者
李莉
杨春萍
商辉
LI Li(Eastern China Conservation Centre for Wild Endangered Plant Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602)
出处
《园艺与种苗》
CAS
2024年第1期15-18,共4页
Horticulture & Seed
基金
国家自然科学基金资助项目(31700170)
中国科学院战略生物资源科技支撑体系运行专项基金资助(ZSZY-001-8)
上海市绿化局科技攻关项目基金资助(G192423和F132412)。
关键词
蕨类植物
孢子培养
交配系统
纯合子
近交衰退
自交系
Fern
Spore propagation
Mating system
Homozygote
Inbreeding depression
Inbred line