不同盐离子胁迫对翅碱蓬幼苗的甜菜红素影响

Effects of Different Salt Ion Stresson Betacyanin in Suaeda salsa Seedlings

[目的]通过对不同浓度的盐离子胁迫处理,检测翅碱蓬幼苗中甜菜红素的变化,对翅碱蓬的生理机制进行初步研究。[方法]通过对翅碱蓬幼苗使用不同浓度的盐离子胁迫处理,利用紫外-可见光分光光度计于538 nm测定吸光度,检测翅碱蓬幼苗中甜菜红素含量的变化。[结果]翅碱蓬幼苗在CaCl_(2)浓度为10 mmol/L时,甜菜红素含量达到最高(12.1 mg/g·FW),但随着CaCl_(2)浓度进一步增加甜菜红素含量减少;翅碱蓬幼苗随着NaCl浓度的增加甜菜红素含量也逐步增加,当浓度达到300 mmol/L时,甜菜红素含量达到11.3 mg/(g·FW);Mg Cl_(2)、KCl随着不同浓度变化,甜菜红素都没有超过对照组。适量的CaCl_(2)、NaCl盐离子胁迫有利于甜菜红素含量的增长,而Mg Cl_(2)、KCl抑制翅碱蓬幼苗中甜菜红素的生成。[结论]可为研究翅碱蓬幼苗中甜菜红素生理合成机理提供参考。

[Objective]The changes of beanthene in the seedlings of Suaeda salsa were detected by different concentration of salt ion stress treatment,and the physiological mechanism of S.salsa was studied.[Method]This study used different concentrations of salt ion stress to treat betacyanin,absorbance was measured at 538 nm by ultraviolet-visible spectrophotometer.[Result]The content of betaine in S.salsa seedlings was determined,when the concentration of CaCl_(2) was 10 mmol/L,the content of betacyanin was the highest(12.1 mg/g·FW),but as the concentration of CaCl further increased,betacyanincontent decreased;as the concentration of NaCl increases,the content of betacyanin gradually increases,when the concentration was higher than 300 mmol/L,the content of betacyanin reached 11.3 mg/(g·FW);MgCl_(2) and KCl change with different concentrations,betacyanin did not exceed the control group,appropriate amount of CaCl_(2) and NaCl salt ion stress is beneficial to the growth of betacyanin content,MgCl_(2) and KCl inhibited the formation of betacyanin in S.salsa seedlings.[Conclusion]It would provide reference for the study of physiological synthesis mechanism of beanthene in S.salsa seedlings.