金银花茎的浸提液对旋覆花的化感作用

Allelopathic effects of the extract from the stem of Lonicera japonica Thunb. on Inula japonica Thunb.

为了研究金银花茎的浸提液对旋覆花幼苗的化感作用及利用化感作用进行生物防除的可能性,论文采用水提法提取金银花茎的浸提液.试验设置0.02、0.04、0.06、0.08 g/mL四个质量浓度梯度,并以蒸馏水为对照组(CK),通过测定旋覆花幼苗的形态与生理指标,研究金银花茎的浸提液对旋覆花的化感效应.结果表明,旋覆花幼苗在金银花茎浸提液的作用下,除旋覆花叶片中的含丙二醛量有所升高外,旋覆花的苗高、根长、苗的干质量以及叶片中过氧化物酶活性等都受到抑制,且随着浸提液质量浓度的升高,抑制作用增强.当质量浓度为0.08 g/mL时,苗高、根长、苗的干生物量及过氧化物酶活性与对照相比分别降低了11.95%、24.95%、31.25%、30.90%.由此可得,金银花茎的浸提液对旋覆花幼苗生长有明显的化感抑制作用,因此其对一定区域的旋覆花幼苗的防除是可行的.

In order to study the allelopathic effect of the extract from the stem of Lonicera japonica Thunb.on the seedlings of Inula japonica Thunb.,and explore the possibility of using this allelopathic effect for biological control.This experiment used the water extraction method to extract the Lonicera japonica Thunb.Stems.Four concentration gradients were set:0.02,0.04,0.06 and0.08 g/mL.Distilled water was used as the control group(CK).The allelopathic effects of the extract of Lonicera japonica Thunb.stems on Inula japonica Thunb.seedlings were studied by measuring their morphological and physiological indicators.The results showed that under the action of the extract of Lonicera japonica Thunb.stem,the seedling height,root length,dry weight,and peroxidase activity in the leaves of Inula japonica Thunb.were inhibited,except for an increase in the content of malondialdehyde in Inula japonica Thunb.Leaves,and the inhibition effect was enhanced with the increase of the concentration of the extract.When the concentration was 0.08 g/mL,the seedling height,root length,dry biomass and peroxidase activity were decreased by 11.95%,24.95%,31.25%and 30.90%respectively compared with the control.It can be concluded that the extract from the stems of Lonicera japonica Thunb.has obvious allelopathic inhibitory effect on the growth of Inula japonica Thunb.seedlings,and it is feasible to control the seedlings of Inula japonica Thunb.in certain areas.