慢性乙型肝炎病毒感染患者CD8^(+)T细胞中LAG-3的表达特征及临床意义

Expression Characteristics and Clinical Significance of LAG-3 in CD8^(+)T Cells from Patients with Chronic Hepatitis B Virus Infection

【目的】探讨慢性乙型肝炎病毒(HBV)感染患者CD8^(+)T细胞中淋巴细胞活化基因-3(LAG-3)的表达特征及临床意义。【方法】选取2019年4月至2020年4月本院收治的60例慢性HBV患者(HBV组),另外选取同期于本院体检的60例健康志愿者作为对照组。比较两组CD8^(+)T细胞中LAG-3表达水平、表达强度、分布频数,并测定细胞内白介素-10(IL-10)、人类白细胞抗原-DR(HLA-DR)、干扰素-γ(IFN-γ)、转录因子T-bet(T-bet)表达水平,分析LAG-3调控CD8^(+)T细胞的机制。【结果】HBV组LAG-3的表达水平、表达强度高于对照组(P<0.05)。HBV组LAG-3阳性亚群的IL-10表达水平高于阴性亚群,且HBV组LAG-3阴性亚群、阳性亚群的IL-10表达水平均高于对照组(P<0.05)。HBV组LAG-3阳性亚群的HLA-DR表达水平低于阴性亚群,且HBV组LAG-3阴性亚群、阳性亚群的HLA-DR表达水平均低于对照组(P<0.05)。HBV组LAG-3阳性亚群的IFN-γ表达水平低于阴性亚群,且HBV组LAG-3阴性亚群、阳性亚群IFN-γ表达水平低于对照组(P<0.05)。HBV组LAG-3阳性亚群的T-bet表达水平低于阴性亚群,且HBV组阴性亚群、阳性亚群IFN-γ表达水平低于对照组(P<0.05)。【结论】慢性HBV感染者在炎症作用下导致CD8^(+)T细胞内LAG-3表达水平增高,而LAG-3能通过影响IL-10、HLA-DR、IFN-γ、T-bet的表达,下调CD8^(+)T对IFN-γ的分泌量,致T细胞消耗量增加。

【Objective】To investigate the expression characteristics and clinical significance of LAG-3 in CD8^(+)T cells from patients with chronic hepatitis B virus(HBV)infection.【Methods】A total of 60 patients with chronic HBV admitted to our hospital from April 2019 to April 2020 were selected as the HBV group.While 60 health examinees in our hospital during the same period were selected as the control group.Blood samples were collected from both groups.The expression level,intensity and frequency of LAG-3 in CD8+T cells were measured by flow cytometry.The expression levels of interleukin-10(IL-10),human leukocyte antigen-DR(HLA-DR),interferon-γ(IFN-γ)and transcription factor T-bet(T-bet)were measured.The mechanism of LAG-3 in regulating CD8^(+)T cells was analyzed.【Results】The expression level and intensity of LAG-3 in the HBV group were higher than those in the control group(P<0.05).The expression of IL-10 in the LAG-3 positive subgroup was higher than that in LAG-3 negative subgroup within HBV group,and the expression of IL-10 in the LAG-3 negative and positive subgroups within HBV group was higher than that in the control group(P<0.05).The expression of HLA-DR in LAG-3 positive subgroup was lower than that in LAG-3 negative subgroup within HBV group,and the expression of HLA-DR in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group(P<0.05).The expression of IFN-gamma in the LAG-3 positive subgroup within HBV group was lower than that in the LAG-3 negative subgroup,and the expression of IFN-gamma in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group(P<0.05).The expression of T-bet in the LAG-3 positive subgroup of HBV group was lower than that in the LAG-3 negative subgroup,and the expression of IFN-gamma in negative and positive subgroups of HBV group was lower than that in the control group(P<0.05).【Conclusion】Chronic HBV infection results in the increase of LAG-3 in CD8^(+)T cells under the action of inflammation.LAG-3 can reduce the secretion of IFN-gamma by affecting the expression of IL-10,HLA-DR,IFN-gamma and T-bet,and increase the consumption of T cells.