单细胞RNA测序应用于继发性肾病的研究进展

Advances in single-cell RNA sequencing in secondary nephrosis

糖尿病肾病、狼疮性肾炎是主要的继发性肾病,是导致终末期肾病的重要原因。探究继发性肾病发生发展过程有利于减少肾功能损害。肾脏细胞具有高度分化且结构复杂的特点,传统测序方法只能探究一类细胞的平均转录水平及关系。近年来,单细胞RNA测序(single-cell RNA-sequencing,scRNA-seq)技术迅猛发展,以高通量及高分辨率对肾组织、血液、尿液细胞样本进行分析,识别新的细胞类型和状态,绘制细胞图谱,从细胞水平维度揭示肾脏疾病的复杂机制,发现生物标志物和细胞特异性。该文就scRNA-seq技术在常见继发性肾病的应用进展进行综述。

Diabetic nephropathy(DN)and lupus nephritis(LN)are the major secondary nephropathy and are important causes of end-stage renal disease(ESRD).Exploring the developmental process of secondary nephrosis is conducive to reducing the renal function damage.Kidney cells have the characteristics of high degree of differentiation and complex structure.Bulk RNA sequencing can only explore the average transcription level and relationship of one type of cells.In recent years,single-cell RNA sequencing(scRNA-seq)technology has developed rapidly.Its high throughput and unprecedented resolution make it possible to analyze the renal tissue,blood,and urine cell samples,to identify the new cell types and states,to draw the cell atlas,to unravel the complex mechanisms of kidney disease from the cellular level dimension,and to discover the biomarkers and cell specificity.This reviews focuses on the latest research advances in scRNA-seq in common secondary nephrosis.