酒精、咖啡、绿茶和乳制品的摄入量与前列腺癌的关联:两样本孟德尔随机化研究

Associations of alcohol,coffee,green tea and dairy products with prostate cancer:a two-sample Mendelian randomization study

目的采用两样本孟德尔随机化方法探究酒精、咖啡、绿茶和乳制品的摄入量与前列腺癌(PCa)发病之间是否存在因果关系,以期帮助明确PCa的发病风险因素,从而帮助寻求PCa的预防途径。方法采用全基因组关联研究(GWAS)收集酒精、咖啡、绿茶、乳制品和PCa的数据,采用两样本孟德尔随机化(2SMR)方法探讨上述饮品摄入量与PCa发病风险的因果关联。逆方差加权法(IVW)是本次孟德尔随机化(MR)分析的主要方法。敏感性分析采用加权中位数、MR-Egger回归、MR多效性残差和离群值(MR-PRESSO)检验。结果咖啡摄入量(OR:0.994,95%CI:0.990~0.999,P=0.014)、绿茶摄入量(OR:0.999,95%CI:0.998~0.999,P=0.036)与PCa发病呈负向因果效应。酒精摄入量(OR:0.997,95%CI:0.990~1.004,P=0.392)和乳制品摄入量(OR:1.025,95%CI:0.983~1.069,P=0.256)与PCa发病风险无因果关联。在加权中位数、MR-Egger回归和留一法分析中,结果均稳健,无异质性和多效性。结论咖啡摄入量和绿茶摄入量与PCa发病存在因果关联,而酒精摄入量与乳制品摄入量和PCa发病则没有因果关联。

Objective A two-sample Mendelian randomization method was used to explore whether there is a causal relationship between the intake of alcohol,coffee,green tea and dairy products and the incidence of prostate cancer(PCa),in order to clarify the risk factors for the incidence of PCa and find a prevention pathway for PCa.Methods Data of alcohol,coffee,green tea,dairy products and prostate cancer were collected with genome-wide association study(GWAS).The causal relationship between their intake and the risk of PCa was analyzed with two-sample Mendelian randomization(2SMR).MR analysis was conducted with inverse-variance weighting(IVW).Sensitivity analysis was performed with weighted median,MR-Egger regression,Mendelian randomization pleiotropy residual sum and outlier(MR-PRESSO)tests.Results Coffee intake(OR:0.994,95%CI:0.990-0.999,P=0.014)and green tea intake(OR:0.999,95%CI:0.998-0.999,P=0.036)were negatively correlated with the risk of PCa.Alcohol intake(OR:0.997,95%CI:0.990-1.004,P=0.392)and dairy intake(OR:1.025,95%CI:0.983-1.069,P=0.256)were not associated with the risk of PCa.In weighted median,MR-Egger regression,and retention one method analyses,the results were robust without heterogeneity or pleiotropy.Conclusion There was a causal association between coffee intake and green tea intake and the onset of PCa,but no causal association between alcohol intake and dairy intake and PCa onset.