土鳖虫雌雄虫对外伤血瘀证大鼠模型的作用差异研究

Study on the Difference of Female and Male Ground Beetles Effect on Traumatic with Blood Stasis Syndrome of Rat Model

目的土鳖虫为伤科要药,临床多以雌虫干燥体入药,本文旨在对比雌雄干燥体对外伤血瘀证的作用差异,为土鳖虫的临床用药提供实验依据。方法将50只雄性Wistar大鼠随机分为5组,每组10只。除正常组外,其余各组大鼠均用重物坠落的方法制备外伤血瘀证模型。采用治疗型给药原则,雌虫组、雄虫组、雌雄虫组大鼠每天灌胃给予雌虫水煎液(1 g生药/kg)、雄虫水煎液(1 g生药/kg)、雌雄虫混合水煎液(1 g生药/kg),正常组和模型组大鼠给予等量纯净水(10 mL/kg)。连续干预5 d后,比较各组大鼠造模前后瘀肿处腿径,采用硝酸还原法(比色法)和放射免疫法检测各组大鼠血清中一氧化氮(nitricoxide,NO)、内皮素(endothelin,ET)-1、血栓素(thromboxane,TX)B2和6-酮-前列腺素(6-keto-protaglandin,6-K-PG)F1α的水平。结果与正常组比较,模型组大鼠瘀肿处腿径增加,血清NO和6-K-PGFlα水平下降,血清ET-1和TXB2水平升高,差异均有统计学意义(P<0.05)。干预后各治疗组大鼠瘀肿处腿径缩小,血清NO、ET-1、TXB2、6-K-PGFlα水平显著回调(P<0.05)。雌虫组、雄虫组和雌雄虫组之间比较,各指标水平差异无统计学意义(P>0.05)。结论土鳖虫雌虫和雄虫对外伤血瘀证均有较好的疗效,且二者的疗效未见明显差异。

Objective The ground beetle is a very important medicine in traumatology department.The female ground beetle is more usually used in clinic.This article is to study the differences between male and female ground beetle in treating the rat model with syndrome of blood stasis by traumatism.Methods 50 male Wistar rats were divided into 5 groups.Except for the normal group,the rats in the remaining groups used the method of falling heavy objects to prepare the traumatic blood stasis syndrome model.According to the principle of therapeutic administration,rats in the female group,male group,and male and female group were given female ground beetle decoction(1 g crude drug/kg),male ground beetle decoction(1 g crude drug/kg),mixed decoction of male and female ground beetle decoction(1 g crude drug/kg),while the rats in the normal group and model group were given equal amounts of purified water(10 mL/kg).After 5 days of continuous intervention,the leg diameters of the bruising areas before and after modeling were compared in each group of rats,and the serum levels of nitricoxide(NO)、endothelin(ET)-1、thromboxane(TX)B2、6-keto-protaglandin(6-K-PG)F1αwere detected by colorimetric method and radioimmunoassay.Results Compare with the normal group,the bruising leg diameter,serum level of ET-1 and TXB2 of the rats in the model group were all higher(P<0.05),while the serum level of NO and 6-K-PGF1αwere decreased in the model group rats(P<0.05).After intervention,the bruising leg diameter rats in each treatment group was reduced,and the levels of serum NO,ET-1,TXB2,and 6-K-PGFlαwere significantly adjusted back(P<0.05).Comparing the female group,the male group and the male and female group,there was no statistically significant difference in the levels of each index(P>0.05).Conclusion Both female and male ground beetle have good curative effects on traumatic blood stasis syndrome,and there is no significant difference in the curative effects between the two.