β-氨基丙腈联合血管紧张素Ⅱ构建胸主动脉瘤小鼠模型的研究

Establishment of a Murine Model of Thoracic Aortic Aneurysm Constructed Viaβ-Aminopropionitrile Combined with AngiotensinⅡ

目的 探讨通过β-氨基丙腈(beta-aminopropionitrile,BAPN)饮水联合Alzet微孔渗透泵皮下连续释放血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)构建胸主动脉瘤小鼠动物模型的方法。方法 60只3周龄雄性C57BL/6小鼠随机平均分为对照组(20只)、实验A组(20只)及实验B组(20只)。对照组、实验A组和实验B组分别采用普通饮水、0.5 g·kg^(-1)·d^(-1)BAPN饮水和1 g·kg^(-1)·d^(-1)BAPN饮水联合微孔渗透泵(Alzet)皮下连续释放Ang Ⅱ(1μg·kg-1·min-1)。监测各组小鼠饮水量及体质量。分别于第30天和第3天采用超声心动图测量小鼠胸主动脉血管内径并计算直径差,取胸主动脉血管组织行病理学检测,评估胸主动脉瘤小鼠动物模型的构建情况。结果 在建模过程中,实验B组中小鼠体质量增长缓慢甚至不增长;实验B组小鼠胸主动脉血管内径差值(d)为(0.519±0.054)mm,相比于对照组(0.135±0.021)mm和实验A组(0.201±0.043)mm,差异有统计学意义(P<0.05);实验A组和实验B组成瘤率分别为5%和40%。结论 本研究成功构建了胸主动脉瘤小鼠动物模型,操作简便、成本低及成功率高,为胸主动脉瘤机制及诊疗靶点的研究奠定了基础。

Objectives To construct a murine model of thoracic aortic aneurysm induced by using of beta-aminopropion-itrile(BAPN)drinking water combined with an Alzet microporous osmotic pump to continuously subcutaneously release angiotensinⅡ(AngⅡ).Methods Sixty 3-week old male C57BL/6 mice were randomly divided into control group(n=20),A experimental group(n=20)and B experimental group(n=20).The mice in control group,A experimental group and B experimental group were fed with ordinary drinking water,BAPN(0.5 g·kg^(-1)·d^(-1))drinking water and BAPN(1 g·kg^(-1)·d^(-1))drinking water,respectively.And then combined with a microporous osmotic pump(Alzet)to continuously subcutaneously release AngⅡ(1μg·kg-1·min-1).The water consumption and body weight of mice in each group were recorded.The inner diameters of the thoracic aorta of mice in each group were measured by echocardiography and the diameter difference was calculated on the 30th and 3rd days.The thoracic aortic vascular tissues were taken for pathological examination to evaluate the construction of the mice model of thoracic aortic aneurysm.Results During the modeling process,the weight of mice in B experimental group increased slowly or even not.The difference of the inner diameter of the thoracic aorta of mice in B experimental group was(0.519±0.054)mm,which was significantly different from control group[(0.135±0.021)mm]and A experimental group[(0.201±0.043 mm)].The aneurysm formation rates of A experimental group and B experimental group were 5%and 40%,respectively.Conclusions In this study,the mice model of thoracic aortic aneurysm was successfully constructed,which was easy to operate,low in cost and high in success rate,which lays a foundation for the study of thoracic aortic aneurysm mechanism and diagnosis and treatment targets.