摘要
Objective:To analyze effects of PRPF18 on the proliferation,migration,invasion,and apoptosis of hepatocellular carcinoma cell line MHCC97H and drug sensitivity analysis.Methods:The expression,prognosis and drug sensitivity of PRPF18 in hepatocellular carcinoma have been analyzed by using bioinformatics.The expression of PRPF18 in HCC and normal hepatocytes was verified by qRT-PCR.PRPF18 was silenced by transient transfection in MHCC97H cells,and the silencing effect was verified by qRT-PCR.CCK-8 assay and colony formation assay were used to analyze the effect of silencing PRPF18 on the proliferation of MHCC97H cells.The effects of silencing PRPF18 on the migration and invasion of MHCC97H cells were analyzed by transwell assay and wound healing assay.Hoechst 33342 staining and mitochondrial membrane potential assay were used to analyze the effect of silencing PRPF18 on apoptosis of MHCC97H cells.Results:PRPF18 is highly expressed in HCC and is significantly associated with the prognosis of patients.Patients with high expression of PRPF18 have higher sensitivity to tipifarnib and sunitinib.After silenced PRPF18,the proliferation,migration,and invasion of MHCC97H cells were significantly lower than those of the control group.Silencing PRPF18 reduced mitochondrial membrane potential and promoted apoptosis.Conclusion:PRPF18 has a good prognostic value in HCC.Silencing PRPF18 may inhibit the proliferation,migration and invasion of MHCC97H cells and promote apoptosis.
基金
Funding for the High-Level Talent Introduction Project of Youjiang Ethnic Medical College(No.yy2021sk002)
Key R&D Project in Qingxiu District,Nanning City(No.2020023)
2021 Guangxi Zhuang Autonomous Region Regional College Student Innovation and Entrepreneurship Training Program Project(No.S202110599073)。
